Involvement of the C-terminal proline-rich motif of G protein-coupled receptor kinases in recognition of activated rhodopsin.

The Journal of Biological Chemistry
Xiaoqing GanLin Li

Abstract

G protein-coupled receptor kinases (GRKs) are a family of serine/threonine kinases that phosphorylate many activated G protein-coupled receptors (GPCRs) and play an important role in GPCR desensitization. Our previous work has demonstrated that the C-terminal conserved region (CC) of GRK-2 participates in interaction with rhodopsin and that this interaction is necessary for GRK-2-mediated receptor phosphorylation (Gan, X. Q., Wang, J. Y., Yang, Q. H., Li, Z., Liu, F., Pei, G., and Li, L. (2000) J. Biol. Chem. 275, 8469-8474). In this report, we further investigated whether the CC of other GRKs had the same functions and defined the specific sequences in CC that are required for the functions. The CC regions of GRK-1, GRK-2, and GRK-5, representatives of the three subfamilies of GRKs, could bind rhodopsin in vitro and inhibit GRK-2-mediated phosphorylation of rhodopsin, but not a peptide GRK substrate. Through a series of mutagenesis analyses, a proline-rich motif in the CC was identified as the key element involved in the interaction between the CC region and rhodopsin. Point mutations of this motif not only disrupted the interaction of GRK-2 with rhodopsin but also abolished the ability of GRK-2 to phosphorylate rhodopsin. The...Continue Reading

References

Jan 1, 1982·Methods in Enzymology·D S Papermaster
Aug 4, 1995·The Journal of Biological Chemistry·T T ChuangA De Blasi
Feb 3, 1995·The Journal of Biological Chemistry·W ShiE R Weiss
Feb 7, 1997·The Journal of Biological Chemistry·A N Pronin, J L Benovic
May 23, 1998·Annual Review of Pharmacology and Toxicology·J G Krupnick, J L Benovic
Aug 1, 1998·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Aug 1, 1998·The Journal of Biological Chemistry·J L FreemanJ A Pitcher
Aug 26, 1998·European Journal of Biochemistry·K HagaH Murofushi
Oct 6, 1998·Annual Review of Biochemistry·J A PitcherR J Lefkowitz
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·R T PremontR J Lefkowitz
Mar 20, 1999·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Nov 24, 1999·The Journal of Biological Chemistry·C V CarmanT Kozasa
Jun 15, 2000·The Journal of Biological Chemistry·A N ProninJ L Benovic
Jul 8, 2000·The Journal of Biological Chemistry·A Ruiz-GómezF Mayor
Jun 5, 2001·The Journal of Biological Chemistry·T ShiinaH Kurose
Jul 30, 2003·Biochemistry·Peter W DayPhilip B Wedegaertner
Dec 5, 2003·Nature·Kristina LorenzUrsula Quitterer

❮ Previous
Next ❯

Citations

Mar 12, 2010·The Journal of Microbiology·Yi-Peng XuChuan-Xi Zhang
Apr 3, 2009·Biochemistry·Rachel Sterne-MarrJohn J G Tesmer
Feb 13, 2008·Photochemistry and Photobiology·Aya TorisawaSatoru Kawamura
May 22, 2009·Journal of Neurochemistry·Konstantin E KomolovKarl-Wilhelm Koch
May 20, 2017·Cell Research·Yuanzheng HeH Eric Xu
Aug 23, 2019·Cellular and Molecular Life Sciences : CMLS·Petronila PenelaFederico Mayor
Jan 18, 2005·The Journal of Biological Chemistry·Christina S Pao, Jeffrey L Benovic

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.