Abstract
G protein-coupled receptor kinases (GRKs) are a family of serine/threonine kinases that phosphorylate many activated G protein-coupled receptors (GPCRs) and play an important role in GPCR desensitization. Our previous work has demonstrated that the C-terminal conserved region (CC) of GRK-2 participates in interaction with rhodopsin and that this interaction is necessary for GRK-2-mediated receptor phosphorylation (Gan, X. Q., Wang, J. Y., Yang, Q. H., Li, Z., Liu, F., Pei, G., and Li, L. (2000) J. Biol. Chem. 275, 8469-8474). In this report, we further investigated whether the CC of other GRKs had the same functions and defined the specific sequences in CC that are required for the functions. The CC regions of GRK-1, GRK-2, and GRK-5, representatives of the three subfamilies of GRKs, could bind rhodopsin in vitro and inhibit GRK-2-mediated phosphorylation of rhodopsin, but not a peptide GRK substrate. Through a series of mutagenesis analyses, a proline-rich motif in the CC was identified as the key element involved in the interaction between the CC region and rhodopsin. Point mutations of this motif not only disrupted the interaction of GRK-2 with rhodopsin but also abolished the ability of GRK-2 to phosphorylate rhodopsin. The...Continue Reading
References
Aug 28, 1992·Science·J A PitcherR J Lefkowitz
Jul 15, 1988·Gene·D B Smith, K S Johnson
Jan 1, 1982·Methods in Enzymology·D S Papermaster
Nov 9, 1982·Biochemistry·P F CookR Roskoski
Aug 4, 1995·The Journal of Biological Chemistry·T T ChuangA De Blasi
Feb 3, 1995·The Journal of Biological Chemistry·W ShiE R Weiss
Feb 7, 1997·The Journal of Biological Chemistry·A N Pronin, J L Benovic
Aug 1, 1997·Protein Expression and Purification·Q H YangL Li
Jun 20, 1998·The Journal of Biological Chemistry·J A PitcherL S Barak
May 23, 1998·Annual Review of Pharmacology and Toxicology·J G Krupnick, J L Benovic
Aug 1, 1998·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Aug 1, 1998·The Journal of Biological Chemistry·J L FreemanJ A Pitcher
Aug 26, 1998·European Journal of Biochemistry·K HagaH Murofushi
Oct 6, 1998·Annual Review of Biochemistry·J A PitcherR J Lefkowitz
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·R T PremontR J Lefkowitz
Mar 20, 1999·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Nov 24, 1999·The Journal of Biological Chemistry·S SarnagoF Mayor
Nov 24, 1999·The Journal of Biological Chemistry·C V CarmanT Kozasa
Nov 27, 1999·The Journal of Biological Chemistry·J A PitcherR J Lefkowitz
Mar 18, 2000·The Journal of Biological Chemistry·X Q GanL Li
Jun 15, 2000·The Journal of Biological Chemistry·A N ProninJ L Benovic
Jul 8, 2000·The Journal of Biological Chemistry·A Ruiz-GómezF Mayor
Mar 22, 2001·The Journal of Biological Chemistry·S V Naga PrasadH A Rockman
Jun 5, 2001·The Journal of Biological Chemistry·T ShiinaH Kurose
Mar 20, 2002·Progress in Neurobiology·Audrey ClaingRobert J Lefkowitz
Oct 16, 2002·Biochemistry·Jennifer L R FreemanRobert J Lefkowitz
Oct 17, 2002·The Journal of Biological Chemistry·Neil J FreedmanKarsten Peppel
Nov 13, 2002·The Journal of Biological Chemistry·Rachel Sterne-MarrPhilip B Wedegaertner
May 24, 2003·Science·David T LodowskiJohn J G Tesmer
May 29, 2003·Chemistry & Biology·Chitose OneyamaKuniaki Tatsuta
Jul 30, 2003·Biochemistry·Peter W DayPhilip B Wedegaertner
Dec 5, 2003·Nature·Kristina LorenzUrsula Quitterer
Citations
Mar 12, 2010·The Journal of Microbiology·Yi-Peng XuChuan-Xi Zhang
Apr 3, 2009·Biochemistry·Rachel Sterne-MarrJohn J G Tesmer
Feb 13, 2008·Photochemistry and Photobiology·Aya TorisawaSatoru Kawamura
Dec 30, 2009·Molecular Pharmacology·Faiza BaameurRichard B Clark
May 22, 2009·Journal of Neurochemistry·Konstantin E KomolovKarl-Wilhelm Koch
May 20, 2017·Cell Research·Yuanzheng HeH Eric Xu
May 5, 2005·The Journal of Biological Chemistry·Gurpreet K DhamiStephen S G Ferguson
Aug 23, 2019·Cellular and Molecular Life Sciences : CMLS·Petronila PenelaFederico Mayor
Mar 7, 2019·Frontiers in Pharmacology·Cristina MurgaFederico Mayor
Jun 25, 2020·Amino Acids·Jeong-Hun KangDaisuke Asai
Apr 21, 2019·Amino Acids·Daisuke AsaiJeong-Hun Kang
Jun 1, 2005·The Journal of Biological Chemistry·Matthias WiensWerner E G Müller
Jan 18, 2005·The Journal of Biological Chemistry·Christina S Pao, Jeffrey L Benovic
May 6, 2006·The Journal of Biological Chemistry·Matthew K HigginsGebhard F X Schertler