Involvement of the contact phase and intrinsic pathway in herpes simplex virus-initiated plasma coagulation

Journal of Thrombosis and Haemostasis : JTH
E S GershomE L G Pryzdial

Abstract

A hemostatic response to vascular injury is initiated by the extrinsic pathway of coagulation and amplified by the intrinsic pathway. We previously reported that purified herpes simplex virus type-1 (HSV1) has constitutive extrinsic pathway tissue factor (TF) and anionic phospholipid on its surface derived from the host cell, and can consequently bypass strict cellular control of coagulation. The current work addresses the hypothesis that HSV1-induced plasma coagulation also involves intrinsic pathway, factor VIII (FVIII), and upstream contact activation pathway, factor XII (FXII). HSV1-initiated clotting was accelerated when purified FVIII was added to FVIII-deficient plasma and in normal plasma attenuated by an inhibitory anti-FVIII antibody (Ab). High HSV1 concentrations predictably reduced the effect of FVIII due to the availability of excess viral TF. To further define TF-independent clotting mechanisms initiated by HSV1, the extrinsic pathway was disabled using factor VII-deficient plasma. The intrinsic pathway is triggered by activation of FXII associated with surface-bound kallikrein, which subsequently activates factor XI. Here we found that an inhibitor of activated FXII, corn trypsin inhibitor, and anti-FXII, anti-ka...Continue Reading

Citations

Apr 25, 2014·Thrombosis Research·Edward L G PryzdialWolfram Ruf
Dec 29, 2015·Journal of Thrombosis and Haemostasis : JTH·Andrew T LongThomas Renné
May 4, 2011·Journal of Thrombosis and Haemostasis : JTH·N J Mutch
Oct 3, 2012·Thrombosis Research·Katrin Faye Nickel, Thomas Renné
Apr 24, 2015·PloS One·Tracy StokolJames Catalfamo
Oct 2, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Maurizio MargaglioneAngela Bruna Maffione
Apr 6, 2018·Frontiers in Medicine·Monika PathakJonas Emsley
Sep 12, 2019·Frontiers in Immunology·Thomas Renné, Evi X Stavrou
Sep 2, 2020·Pharmaceuticals·Ana Paula A DagninoRodrigo B M Silva
Mar 15, 2018·Frontiers in Immunology·Sonja Oehmcke-Hecht, Juliane Köhler
Aug 28, 2020·Frontiers in Immunology·Simone MeiniCarlo Tascini
Mar 17, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Huan HanCheng-Liang Zhu
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hassan MadkhaliZia Shariat-Madar

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