Involvement of the cysteine protease BcAtg4 in development and virulence of Botrytis cinerea

Current Genetics
Na LiuZhonghua Ma

Abstract

Autophagy serves as a survival mechanism against starvation and has been reported to be important for cell growth and differentiation in eukaryotes. Here, we investigated the function of a cysteine protease BcAtg4 in the gray mold fungus Botrytis cinerea. Yeast complementation experiments revealed that Bcatg4 can functionally replace the counterpart of yeast. Subcellular localization exhibited that BcAtg4 diffused in cytoplasm at different developmental stages. Targeted gene deletion of Bcatg4 (ΔBcatg4) led to autophagy blocking and a significant retardation in growth and conidiation. In addition, ΔBcatg4 failed to form sclerotia. Infection tests demonstrated that ΔBcatg4 was severely attenuated in virulence on different host plant tissues. All of the phenotypic defects were restored by reintroducing an intact copy of Bcatg4 into ΔBcatg4. These results indicate that Bcatg4 plays multiple roles in the developmental processes and pathogenesis of B. cinerea.

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Citations

Jun 12, 2019·Current Genetics·Wenqing GuanTingheng Zhu
Jan 23, 2020·International Journal of Molecular Sciences·Xinqiang LiuJiasen Cheng
Apr 7, 2020·PloS One·Thiago Nunes RobertoMarcelo Afonso Vallim
Feb 26, 2021·Journal of Experimental Botany·Rita B Santos, Andreia Figueiredo

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Methods Mentioned

BETA
genetic modifications
PCR

Software Mentioned

MEGA

Related Concepts

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