Involvement of the cytoskeleton in oxytocin secretion by cultured bovine luteal cells

Biology of Reproduction
Masami ShibayaDariusz J Skarzynski

Abstract

A number of substances have been implicated in the regulation of oxytocin (OT) secretion from bovine corpus luteum in vivo. However, isolated bovine luteal cells cultured in a monolayer lose the ability to secrete OT in response to stimulatory substances. The present study investigated how cell-to-cell contact and the cytoskeleton affect OT secretion by isolated bovine luteal cells. In experiment 1, bovine midluteal cells (Days 8-12 of the estrous cycle) were stimulated with prostaglandin F2alpha (PGF2alpha; 1 microM), noradrenaline (NA; 10 microM), or growth hormone (GH; 5 nM) in two culture systems: In one system, cell monolayers were incubated in 24-well culture plates, and in the other system, aggregates of cells were incubated in glass tubes in a shaking water bath. The cells cultured in a monolayer underwent considerable spreading and showed a variety of shapes, whereas the cells cultured in glass tubes remained fully rounded during the experimental period and soon formed aggregates of cells. Although PGF2alpha, NA, and GH did not stimulate OT secretion by the monolayer cells, all tested substances stimulated OT secretion by the aggregated cells (P < 0.01). In experiment 2, the monolayer cells were pre-exposed for 1 h to ...Continue Reading

References

Jan 1, 1977·Journal of Reproduction and Fertility·R T Gemmell, R D Stacy
Jan 1, 1976·Annual Review of Biochemistry·J A Snyder, J R McIntosh
Jun 1, 1992·Biology of Reproduction·K OkudaD Schams
Jan 1, 1987·Journal of Steroid Biochemistry·A R Fuchs
May 1, 1988·Journal of Reproduction and Fertility·B K TsangJ A Carnegie
Oct 1, 1987·Biology of Reproduction·S E AbdelgadirF Stormshak
Oct 14, 1985·FEBS Letters·R IvellD Richter
Sep 3, 1984·FEBS Letters·R W SwannB T Pickering
Jan 1, 1995·Domestic Animal Endocrinology·R P Del VecchioW Hansel
Jan 1, 1996·International Review of Cytology·A De LoofI Janssen
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa
Feb 28, 2003·Biology of Reproduction·Dariusz J SkarzynskiWilliam Hansel
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Fredrick Stormshak

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