PMID: 8970959Dec 1, 1996Paper

Involvement of the endoplasmic reticulum in the assembly and envelopment of African swine fever virus

Journal of Virology
C CobboldThomas Wileman

Abstract

African swine fever (ASF) virus is a large enveloped DNA virus assembled in the cytoplasm of cells. In this study, the membrane compartments involved in the envelopment of ASF virus were investigated. A monoclonal antibody recognizing p73, the major structural protein of ASF virus, was generated to analyze the binding of p73 to membranes during the assembly of the virus. Approximately 50% of the intracellular pool of p73 associated with membranes as a peripheral membrane protein. Binding was rapid and complete within 15 min of synthesis. Subcellular membrane fractionation showed that newly synthesized p73 molecules cosedimented with endoplasmic reticulum (ER) membranes and remained associated with the ER during a 2-h chase. A similar distribution on gradients was recorded for p17, a structural membrane protein of ASF virus. The results suggested that the ER was involved in the assembly of ASF virus. A protease protection assay demonstrated a time-dependent envelopment of the membrane bound, but not cytosolic, pool of p73. Envelopment of p73 took place 1 h after binding to membranes and was completed 1 h before the first detection of p73 in virions secreted from cells. Envelopment was unaffected by brefeldin A and monensin, drug...Continue Reading

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Citations

Nov 20, 2002·Journal of Virology·Germán AndrésMaría L Salas
Jul 28, 2006·Cellular Microbiology·Nolwenn JouvenetThomas Wileman
Nov 18, 2003·Annual Review of Genetics·James L Van Etten
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine A WellsRobert B Ashman
Dec 26, 2008·Journal of Virology·Christopher L NethertonGeraldine Taylor
Jan 21, 2011·Journal of Virology·Lynnette C Goatley, Linda K Dixon
Jun 14, 2008·Journal of Virology·Philippa C HawesPaul Monaghan
May 2, 2001·The Journal of Cell Biology·C M HeathT Wileman
Mar 14, 1998·Journal of Virology·I RouillerT Wileman
Jun 8, 2017·Viruses·Miguel Ángel Cuesta-GeijoCovadonga Alonso
Dec 16, 2006·The Journal of General Virology·Christian CobboldThomas Wileman
Jan 13, 2021·Viruses·Sophie-Marie AicherPhilippa C Hawes
Jan 5, 2002·Trends in Microbiology·Beate Sodeik, Jacomine Krijnse-Locker
Dec 17, 2003·The Journal of Investigative Dermatology·Jittima DhitavatAlain Hovnanian
Jul 18, 2006·Molecular Genetics and Metabolism·Christian CobboldMichael Badminton

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