Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses

Frontiers in Cellular and Infection Microbiology
Lu WangQingpi Yan

Abstract

Adhesion is an important virulence factor of Vibrio alginolyticus. This factor may be affected by environmental conditions; however, its molecular mechanism remains unclear. In our previous research, adhesion deficient strains were obtained by culturing V. alginolyticus under stresses including Cu, Pb, Hg, and low pH. With RNA-seq and bioinformatics analysis, we found that all of these stress treatments significantly affected the flagellar assembly pathway, which may play an important role in V. alginolyticus adhesion. Therefore, we hypothesized that the environmental stresses of the flagellar assembly pathway may be one way in which environmental conditions affect adhesion. To verify our hypothesis, a bioinformatics analysis, QPCR, RNAi, in vitro adhesion assay and motility assay were performed. Our results indicated that (1) the flagellar assembly pathway was sensitive to environmental stresses, (2) the flagellar assembly pathway played an important role in V. alginolyticus adhesion, and (3) motility is not the only way in which the flagellar assembly pathway affects adhesion.

References

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Citations

Jan 27, 2018·Genome Announcements·Lixing HuangQingpi Yan
May 17, 2019·International Journal of Molecular Sciences·Shulin FuChien-An Andy Hu
May 22, 2020·Critical Reviews in Food Science and Nutrition·Md AshrafudoullaSang-Do Ha
Sep 30, 2020·Applied Microbiology and Biotechnology·Lingjuan LiYong Tao
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Xiaojin XuXinglong Jiang

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Datasets Mentioned

BETA
SRP049226

Methods Mentioned

BETA
RNA-seq
environmental stresses
motility
scraping
environmental
motility assays
motility assay

Software Mentioned

Osiris
WEGO
Blastall
SPSS
Blast2GO

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