Involvement of the global regulator H-NS in the survival of Escherichia coli in stationary phase.

Journal of Bacteriology
Savita Chib, S Mahadevan

Abstract

Long-term batch cultures of Escherichia coli grown in nutrient-rich medium accumulate mutations that provide a growth advantage in the stationary phase (GASP). We have examined the survivors of prolonged stationary phase to identify loci involved in conferring a growth advantage and show that a mutation in the hns gene causing reduced activity of the global regulator H-NS confers a GASP phenotype under specific conditions. The hns-66 allele bears a point mutation within the termination codon of the H-NS open reading frame, resulting in a longer protein that is partially functional. Although isolated from a long-term stationary-phase culture of the parent carrying the rpoS819 allele that results in reduced RpoS activity, the hns-66 survivor showed a growth disadvantage in the early stationary phase (24 to 48 h) when competed against the parent. The hns-66 mutant is also unstable and reverts at a high frequency in the early stationary phase by accumulating second-site suppressor mutations within the ssrA gene involved in targeting aberrant proteins for proteolysis. The mutant was more stable and showed a moderate growth advantage in combination with the rpoS819 allele when competed against a 21-day-old parent. These studies show ...Continue Reading

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Citations

May 11, 2013·Molecular BioSystems·Rajalakshmi SrinivasanAswin Sai Narain Seshasayee
Nov 29, 2014·Nucleic Acids Research·Rajalakshmi SrinivasanAswin Sai Narain Seshasayee
May 5, 2017·Molecular Microbiology·Ashley ParkerSusan Gottesman
Feb 24, 2015·G3 : Genes - Genomes - Genetics·Elisa Brambilla, Bianca Sclavi
Jul 15, 2021·IScience·Ronen HazanMichael Klutstein

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