Involvement of the lamin rod domain in heterotypic lamin interactions important for nuclear organization

The Journal of Cell Biology
Eric C SchirmerL Gerace

Abstract

The nuclear lamina is a meshwork of intermediate-type filament proteins (lamins) that lines the inner nuclear membrane. The lamina is proposed to be an important determinant of nuclear structure, but there has been little direct testing of this idea. To investigate lamina functions, we have characterized a novel lamin B1 mutant lacking the middle approximately 4/5 of its alpha-helical rod domain. Though retaining only 10 heptads of the rod, this mutant assembles into intermediate filament-like structures in vitro. When expressed in cultured cells, it concentrates in patches at the nuclear envelope. Concurrently, endogenous lamins shift from a uniform to a patchy distribution and lose their complete colocalization, and nuclei become highly lobulated. In vitro binding studies suggest that the internal rod region is important for heterotypic associations of lamin B1, which in turn are required for proper organization of the lamina. Accompanying the changes in lamina structure induced by expression of the mutant, nuclear pore complexes and integral membrane proteins of the inner membrane cluster, principally at the patches of endogenous lamins. Considered together, these data indicate that lamins play a major role in organizing oth...Continue Reading

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Citations

Jan 22, 2010·Cellular and Molecular Life Sciences : CMLS·Poonam MalikEric C Schirmer
Nov 12, 2005·Journal of Molecular Histology·Darran TunnahGlenn E Morris
Jun 18, 2002·Current Opinion in Cell Biology·James M HolaskaMalini Mansharamani
Dec 20, 2002·Journal of Structural Biology·Christine K DregerHarald Herrmann
Nov 2, 2004·Nature Cell Biology·Chris J Hutchison, Howard J Worman
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Yosef GruenbaumKatherine L Wilson
Sep 30, 2010·Molecular & Cellular Proteomics : MCP·Gavin S WilkieEric C Schirmer
Oct 1, 2003·Molecular Biology of the Cell·Merav CohenYosef Gruenbaum
Jan 7, 2005·Molecular Biology of the Cell·Thomas A Lagace, Neale D Ridgway
Feb 3, 2006·Genes & Development·Carmen IvorraVicente Andrés
May 28, 2004·Journal of Virology·Cristen C HoytKenneth L Tyler
Sep 19, 2012·The Journal of Cell Biology·Moritz MallIain W Mattaj
Feb 13, 2002·The Journal of Cell Biology·Phyllis FrosstLarry Gerace
Feb 9, 2012·Seminars in Cancer Biology·Jose I de Las HerasEric C Schirmer
Mar 2, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lu Sui, Yanhong Yang
Aug 31, 2004·Reproductive Biomedicine Online·Gary D Smith, Cristine Ane Silva E Silva
Sep 1, 2009·Biochemical and Biophysical Research Communications·Cecilia OstlundEric C Schirmer
Feb 13, 2009·Experimental Cell Research·Kazuhiro FurukawaShin Sugiyama
Aug 30, 2008·Mutation Research·Eric C Schirmer
Apr 25, 2007·Experimental Cell Research·Eric C Schirmer, Roland Foisner
May 12, 2007·Experimental Cell Research·Colin L StewartStephen G Young
May 10, 2007·The FEBS Journal·Sigal ShaklaiAmos J Simon
Nov 14, 2012·EMBO Reports·Noam ZuelaYosef Gruenbaum
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Mar 8, 2005·Experimental Cell Research·Yuji Nakayama, Naoto Yamaguchi
Aug 30, 2005·Trends in Biochemical Sciences·Eric C Schirmer, Larry Gerace
Aug 29, 2006·Biochimica Et Biophysica Acta·Sylvia Vlcek, Roland Foisner
Oct 20, 2006·Biochimica Et Biophysica Acta·F HoubenJ L V Broers
Oct 17, 2013·Plant Signaling & Behavior·Malgorzata Ciska, Susana Moreno Diaz de la Espina
Oct 6, 2011·Nucleus·Nikolaj ZulegerEric C Schirmer
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Nov 12, 2014·PloS One·Poonam MalikEric C Schirmer
Apr 9, 2008·European Journal of Cell Biology·Gabriela GaliováStanislav Kozubek
Feb 28, 2017·Nature·Yagmur TurgayOhad Medalia
Apr 9, 2005·Pediatric Research·Raz SomechAmos J Simon

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Datasets Mentioned

BETA
M94362
but

Methods Mentioned

BETA
light microscopy
PCR
transfection
FACS
circular dichroism
fluorescence microscopy

Software Mentioned

DeltaVision
Photoshop
OMNIC

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