Abstract
Exposure to 100% oxygen causes hyperoxic acute lung injury characterized by cell death and injury of alveolar epithelial cells. Recently, the role of chitinase 3-like 1 (CHI3L1), a member of the glycosyl hydrolase 18 family that lacks chitinase activity, in oxidative stress was demonstrated in murine models. High levels of serum CHI3L1 have been associated with various diseases of the lung, such as asthma, chronic obstructive pulmonary disease, and cancer. However, the role of CHI3L1 in human airway epithelial cells undergoing oxidative stress remains unknown. In addition, the signaling pathways associated with CHI3L1 in this process are poorly understood. In this study, we demonstrate the role of CHI3L1, along with the MAPK and PI3K signaling pathways, in hyperoxia-exposed airway epithelial cells. The human airway epithelial cell line, BEAS-2B, was exposed to >95% oxygen (hyperoxia) for up to 72h. Hyperoxia-induced cell death was determined by assessing cell viability, Annexin-V FITC staining, caspase-3 and -7 expression, and electron microscopy. CHI3L1 knockdown and overexpression studies were conducted in BEAS-2B cells to examine the role of CHI3L1 in hyperoxia-induced apoptosis. Activation of the MAPK and PI3K pathways was ...Continue Reading
References
Mar 1, 1993·Free Radical Biology & Medicine·M A CacciuttoloG Rao
Jun 21, 1996·The Journal of Biological Chemistry·J A KazzazS Horowitz
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·C BarazzoneP F Piguet
Aug 18, 2001·American Journal of Respiratory Cell and Molecular Biology·S A McGrath-Morrow, J Stahl
Mar 6, 2002·The Journal of Biological Chemistry·G R Scott BudingerNavdeep S Chandel
Jun 20, 2002·The Biochemical Journal·Anneliese D ReckliesHua Ling
Sep 9, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Monique E De PaepeSurendra Sharma
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
Feb 24, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Julia S JohansenPaul A Price
Sep 7, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C N Rathcke, H Vestergaard
Sep 16, 2006·Cellular & Molecular Biology Letters·Indrajit ChowdhuryGanapathy K Bhat
Mar 9, 2007·Scandinavian Journal of Rheumatology·L S KnudsenJ S Johansen
Nov 16, 2007·The New England Journal of Medicine·Geoffrey L ChuppJack A Elias
Apr 12, 2008·The New England Journal of Medicine·Carole OberGeoffrey L Chupp
Apr 17, 2008·Nature Reviews. Molecular Cell Biology·Alexei Degterev, Junying Yuan
Jul 11, 2008·Scandinavian Cardiovascular Journal : SCJ·Yongzhong WangJens Kastrup
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·John G WalshSeamus J Martin
Mar 30, 2010·American Journal of Respiratory Cell and Molecular Biology·Rajesh S AlphonseBernard Thébaud
Jun 19, 2010·American Journal of Respiratory and Critical Care Medicine·Myung Hyun SohnJack A Elias
Jul 24, 2010·The Journal of Biological Chemistry·Jin-Ah ParkDaniel J Tschumperlin
Jul 27, 2010·American Journal of Respiratory Cell and Molecular Biology·Hiroshi MatsuuraChun Geun Lee
Sep 3, 2010·Pediatric Pulmonology·Yan ChenRui Pan
Citations
Jan 1, 2014·BMC Pulmonary Medicine·Dennis B HolmgaardCourt Pedersen
Dec 18, 2013·Cancer Metastasis Reviews·Kartik GuptaRajan Gogna
Apr 8, 2015·Molecular Medicine Reports·Tie LiuGang Liu
Jan 1, 2013·Trends in Cell Biology·George T Eisenhoffer, Jody Rosenblatt
May 9, 2015·American Journal of Respiratory Cell and Molecular Biology·Min-Jong KangJack A Elias
Jun 30, 2015·The Journal of Clinical Investigation·Yang ZhouJack A Elias
Dec 3, 2014·Cytotherapy·Philipp NoldCornelia Brendel
Aug 27, 2013·Cell Reports·Chuan Hua HeJack A Elias
Jan 30, 2015·American Journal of Respiratory and Critical Care Medicine·Farida AhangariJack A Elias
Dec 9, 2014·Respiratory Physiology & Neurobiology·Andrea PorzionatoCamillo Di Giulio
Aug 22, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jian-Ping ZhangJian-Ming Guo
Oct 4, 2016·Respiratory Medicine·Francisca Dias-FreitasRoberto Roncon-Albuquerque
May 18, 2016·Critical Reviews in Clinical Laboratory Sciences·A D KjaergaardB G Nordestgaard
May 21, 2016·Scientific Reports·Bing MaJack A Elias
Dec 2, 2017·American Journal of Reproductive Immunology : AJRI·Robert M Dietz, Clyde J Wright
Jul 19, 2014·Anti-cancer Drugs·Xiao-Min QiuJia-He Wang
Aug 10, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Eun Ji KwakMyung Hyun Sohn
Mar 4, 2020·International Journal of Molecular Medicine·Ping HeCheng Wang
May 28, 2020·American Journal of Respiratory Cell and Molecular Biology·Alane B C Dy, Julie G Ledford
Jun 21, 2013·Molecular Medicine Reports·Qiuhua ChenHaibo Qiu
Feb 11, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yang ZhouJack A Elias
Jan 20, 2017·Oncology Letters·Zhiwu LiuBaojin Chen
Sep 17, 2020·Allergy, Asthma & Immunology Research·Eun Gyul KimMyung Hyun Sohn
Jan 28, 2021·Cells·Songhua ZhangJack R Wands
Mar 22, 2021·Biochemical and Biophysical Research Communications·Ze-Xin LiShui-Jun Zhang
May 8, 2021·The Journal of Pathology·Sharif MoradiMostafa Ghanei
Sep 2, 2021·Journal of Inflammation Research·Yang SongBin Chen