Involvement of the matrix protein in attachment of porcine reproductive and respiratory syndrome virus to a heparinlike receptor on porcine alveolar macrophages

Journal of Virology
Peter L DelputteM B Pensaert

Abstract

The porcine reproductive and respiratory syndrome virus (PRRSV) has a very restricted tropism for well-differentiated cells of the monocyte-macrophage lineage, which is probably determined by specific receptors on these cells. In this study, the importance of heparinlike molecules on porcine alveolar macrophages (PAM) for PRRSV infection was determined. Heparin interacted with the virus and reduced infection of PAM up to 92 or 88% for the American and European types of PRRSV, respectively. Other glycosaminoglycans, similar to heparin, had no significant effect on infection while heparinase treatment of PAM resulted in a significant reduction of the infection. Analysis of infection kinetics showed that PRRSV attachment to heparan sulfate occurs early in infection. A heparin-sensitive binding step was observed which converted completely into a heparin-resistant binding after 120 min at 4 degrees C. Using heparin-affinity chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), it was observed that the structural matrix (M) and nucleocapsid (N) proteins attached to heparin. Nonreducing SDS-PAGE revealed that M bound to heparin mainly as a complex with glycoprotein GP(5) and that the N protein bound ...Continue Reading

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J E CollinsH S Joo
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D A BenfieldD Chladek
Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
Jul 1, 1988·Journal of Virology·C SchreursT Ben-Porat
Jan 10, 1995·Virology·J J MeulenbergG Wensvoort
May 29, 1998·The Journal of General Virology·E J Snijder, J J Meulenberg
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R E HilemanR J Linhardt
Aug 8, 1998·Journal of Virology·A P Byrnes, D E Griffin
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Apr 1, 1959·Virology·A S KAPLAN, A E VATTER

❮ Previous
Next ❯

Citations

Jan 5, 2014·The Journal of Microbiology·Sang-Im Yun, Young-Min Lee
Mar 28, 2012·Seminars in Immunopathology·Mariliis Klaas, Paul R Crocker
Apr 25, 2008·The Journal of Biological Chemistry·Edward N HarrisPaul H Weigel
Sep 26, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P L DelputteH J Nauwynck
May 12, 2007·Journal of Virology·Jay G CalvertSiao-Kun W Welch
Oct 19, 2007·Journal of Virology·Prasad MulupuriMichael P Murtaugh
Dec 13, 2002·Journal of Virology·Eric J SnijderWilly J M Spaan
Jul 16, 2004·Journal of Virology·Peter L Delputte, Hans J Nauwynck
May 29, 2013·Research in Veterinary Science·Hsin-Ping YangChao-Wei Liao
Jul 27, 2010·Virus Research·Siao-Kun W Welch, Jay G Calvert
Aug 10, 2010·Virus Research·Terje Dokland
May 26, 2009·Vaccine·Tjeerd G KimmanNorbert Stockhofe-Zurwieden
May 30, 2009·Veterinary Microbiology·Ping an XiaPu yan Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.