Involvement of the rapid increase in cAMP content in the vanadate-stimulated release of lipoprotein lipase activity from rat fat pads

Biological & Pharmaceutical Bulletin
T MotoyashikiH Ueki

Abstract

Mechanisms of the stimulatory release of lipoprotein lipase (LPL) activity from isolated rat fat pads by sodium orthovanadate (vanadate) were studied through a cAMP-dependent process. A potent inhibitor of insulin receptor tyrosine kinase, quercetin, inhibited the vanadate-increasing effect on the LPL activity in fat pads, but did not inhibit the vanadate-stimulated release of LPL activity from the fat pads. Propranolol and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) decreased the vanadate-stimulated release in a dose-dependent manner. Isoproterenol and dibutyryl cAMP (Bt2cAMP) stimulated the release of LPL activity from fat pads. Vanadate, as well as isoproterenol, rapidly increased the cAMP content in fat pads, and this increase was almost completely inhibited by propranolol. Vanadate increased the cAMP-dependent protein kinase (PKA) activity ratios calculated from the measurement in the presence or absence of cAMP or PKa inhibitor. These results suggest that the vanadate-stimulated release of LPL activity is associated with a process involving a rapid increase in the cAMP content accompanied by the activation of PKA.

Citations

Feb 4, 2011·Phytotherapy Research : PTR·Yeon-Mi YangJeong-Chae Lee
Oct 4, 2002·Archives of Biochemistry and Biophysics·Aya YoshidaHiroshi Ueki
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Aya Suenaga, Hiroshi Ueki
Apr 18, 2001·Biological & Pharmaceutical Bulletin·T SuenagaH Ueki
Aug 23, 2003·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroshi Ueki
Dec 16, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·M B ElamW P Forbes
Nov 16, 2017·Oxidative Medicine and Cellular Longevity·Yueshui ZhaoZhangang Xiao
Feb 24, 2001·The Annals of Pharmacotherapy·M P Reilly, E R Mohler
Oct 30, 2020·Pharmaceuticals·María José García-BarradoJosé Carretero
Jul 18, 2021·Journal of Ethnopharmacology·Anuj Kumar BorahJagat Chandra Borah

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