PMID: 7543586Sep 1, 1995Paper

Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 epitopes induced by receptor binding

Journal of Virology
R WyattJ Sodroski

Abstract

The binding of human immunodeficiency virus type 1 (HIV-1) to the cellular receptor CD4 has been suggested to induce conformational changes in the viral envelope glycoproteins that promote virus entry. Conserved, discontinuous epitopes on the HIV-1 gp120 glycoprotein recognized by the 17b, 48d, and A32 antibodies are preferentially exposed upon the binding of soluble CD4 (sCD4). The binding of the 17b and 48d antibodies to the gp120 glycoprotein can also be enhanced by the binding of the A32 antibody. Here we constructed HIV-1 gp120 mutants in which the variable segments of the V1/V2 and V3 structures were deleted, individually or in combination, while the 17b, 48d, and A32 epitopes were retained. The effects of the variable loop deletions on the function of the HIV-1 envelope glycoproteins and on the exposure of epitopes induced by sCD4 or A32 binding to the monomeric gp120 glycoprotein were examined. The variable-loop-deleted envelope glycoproteins were able to mediate virus entry, albeit at lower efficiencies than those of the wild-type glycoproteins. Thus, the V1/V2 and V3 variable sequences contribute to the efficiency of HIV-1 entry but are not absolutely required for the process. Neither the V1/V2 nor V3 loops were neces...Continue Reading

References

Apr 1, 1992·Journal of Virology·P WesterveltL Ratner
Aug 1, 1991·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D D HoN C Sun
Jul 11, 1991·AIDS Research and Human Retroviruses·L A IvanoffT J Matthews
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T K HartP J Bugelski
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P WesterveltL Ratner
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C Y KangH Köhler
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E O FreedR Risser
Nov 23, 1990·Science·J P MooreQ J Sattentau
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E S DaarD D Ho
Jul 18, 1985·Nature·A G FisherF Wong-Staal
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R RuscheP J Fischinger
Feb 9, 1989·Nature·D J CaponJ E Groopman
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K JavaherianS D Putney
Jan 7, 1988·Nature·R A FisherR T Schooley
Jan 7, 1988·Nature·A TrauneckerK Karjalainen

❮ Previous
Next ❯

Citations

Mar 25, 2004·Nature Reviews. Immunology·Susan Zolla-Pazner
May 4, 2005·The Journal of Experimental Medicine·Julie M DeckerGeorge M Shaw
Oct 6, 2006·Springer Seminars in Immunopathology·Shan Lu
Jul 11, 2006·Expert Review of Vaccines·Barton F Haynes, David C Montefiori
Feb 21, 2008·AIDS Research and Human Retroviruses·Suganya SelvarajahDennis R Burton
Jan 6, 2010·Current Opinion in HIV and AIDS·Penny L MooreLynn Morris
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shang-Rung WuHenrik Garoff
Feb 10, 2011·Journal of Translational Medicine·Claudia CicalaAnthony S Fauci
Dec 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yongjun GuanGeorge K Lewis
Mar 27, 2015·Journal of Virology·Marina R AlexanderPer Johan Klasse

❮ Previous
Next ❯