Involvement of the VEGF signaling pathway in immunosuppression and hypoxia stress: analysis of mRNA expression in lymphocytes mediating panting in Jersey cattle under heat stress.

BMC Veterinary Research
Jian WangMonchai Duangjinda

Abstract

Extreme panting under heat stress threatens dairy cattle milk production. Previous research has revealed that the gas exchange-mediated respiratory drive in critically ill dairy cattle with low O2 saturation induces panting. Vascular endothelial growth factor (VEGF) signaling may play important roles in immunosuppression and oxidative stress during severe respiratory stress responses in heat-stressed cattle. The objectives of this study were to transcriptomically analyze mRNA expression mediating heat-induced respiratory stress-associated panting, evaluate gas exchange, screen hub genes, and verify the expression of proteins encoded by differentially expressed genes in lymphocyte pathways. Jersey cattle were naturally heat-exposed. Physiological data were collected for response evaluation, and blood was collected for gas exchange and gene expression assays at 06:00, 10:00 and 14:00 continuously for 1 week. Lymphocytes were isolated from whole-blood samples for mRNA-seq and expression analysis of key pathway genes/proteins. The cattle respiration rates differed with time, averaging 51 bpm at 06:00, 76 bpm at 10:00, and 121 bpm at 14:00 (p < 0.05). Gas exchange analysis showed that both pH and pCO2 differed with time: they were 7...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Nov 7, 2006·Journal of Animal Science·J L Salak-Johnson, J J McGlone
Mar 21, 2007·Journal of Dairy Science·J BohmanovaJ B Cole
Mar 28, 2008·American Journal of Respiratory Cell and Molecular Biology·Ishwar S SinghJeffrey D Hasday
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yoshimitsu DoiTakashi Yamamura
Nov 7, 2008·Nature Reviews. Cancer·Jessica A BertoutM Celeste Simon
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Sep 29, 2009·Trends in Immunology·Subhra K Biswas, Eduardo Lopez-Collazo
Oct 11, 2012·North American Journal of Medical Sciences·Tahrin Mahmood, Ping-Chang Yang
Jul 5, 2013·International Journal of Biometeorology·Wang JianSuporn Katawatin
Feb 27, 2015·Journal of Inflammation·Joseph M McCoyKimberlee S Mix
Apr 22, 2015·Cell·Dong Chul LeeYoung Il Yeom
Aug 8, 2015·Journal of Animal Physiology and Animal Nutrition·I Belhadj SlimenM Abdrrabba
Jan 25, 2017·Journal of Food Science·Huijuan ZhangBaoguo Sun
Apr 7, 2019·Seminars in Immunopathology·Motoko Y KimuraToshinori Nakayama
Aug 24, 2019·Frontiers in Pharmacology·Xiao-Mian LinXiao-Ping Wu
Nov 18, 2019·Endocrine Regulations·Dmytro O MinchenkoOleksandr H Minchenko

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ImageJ
Image Lab
SAS
GO R package
DESeq2
KOBAS
Database for Annotation , Visualization and Integrated Discove...
GOATOOLS
RSEM

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