Involvement of TNF-α and MAPK pathway in the intramammary MMP-9 release via degranulation of cow neutrophils during acute mammary gland involution

Veterinary Immunology and Immunopathology
Ting-Chieh YuChai-Ju Chang

Abstract

Neutrophil-derived MMP-9 activity is regulated more promptly and efficiently at the level of degranulation than at other levels of regulation. In human neutrophils, degranulation is one of the earliest responses to TNF-α stimulation, which involves protein kinase C and mitogen-activating protein kinase (MAPK) pathways. The level of MMP-9 in mammary secretion of cows increases drastically following milk-stasis, which is partially explained by increases of both neutrophil infiltration and neutrophil degranulation per se. Since MMP-9 represents one of the major remodeling capacities in the mammary gland of cows during early dry period, the current study attempted to explore the involved intracellular mechanisms in the up-regulated MMP-9 secretion. We repeatedly measured on the somatic cells of mammary secretion along the early dry period of cows the expression of TNF-α protein and the phosphorylation of p38 MAPK, ERK, and JNK. Also, cultures of bovine peripheral neutrophils were conducted to examine the mode of short-term MMP-9 secretion in response to TNF-α stimulation and the blocking effects of TNF-α antibody and inhibitors of MAPK pathways. Ex vivo measurements show that conventional cow milk has fully transformed into a neutr...Continue Reading

References

Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·J PuginA F Suffredini
Jan 24, 2003·Critical Reviews in Biochemistry and Molecular Biology·Philippe E Van den SteenGhislain Opdenakker
Apr 16, 2005·Journal of Leukocyte Biology·Subhadeep Chakrabarti, Kamala D Patel
Aug 5, 2005·Journal of Cell Science·Roberto MontesanoFabio Carrozzino
Nov 12, 2005·Science·Rachael Z MurrayJennifer L Stow
Mar 14, 2008·Toxicology and Applied Pharmacology·Chih-Chung LinChuen-Mao Yang
Sep 9, 2010·The Journal of Dairy Research·Chin H HoHajime Nagahata

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Citations

Oct 13, 2014·Journal of Dairy Science·A DomènechA Arís
Oct 18, 2014·Animal : an International Journal of Animal Bioscience·P PiamyaC-J Chang
Nov 6, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nicholas H McCormickShannon L Kelleher

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