Involvement of transcriptional enhancers in the regulation of developmental expression of yellow gene

Science in China. Series C, Life Sciences
J ChenZ Jin

Abstract

Upstream regulatory region and flanking DNA of yellow gene were isolated and cloned from a Drosophila genomic library. A vector containing yellow gene and regulatory elements was constructed using the recombinant DNA technique. Then this vector was integrated into Drosophila genome by genetic transformation. Using both FLP/FRT and Cre/LoxP site-specific recombination systems, two new yellow alleles were created at the same position in the genome of transgenic flies. Results from genetic and molecular analysis indicated that transcriptional enhancers regulate the developmental expression of the transgene. Furthermore, interactions between new-created yellow alleles were observed. Such interactions can influence markedly the expression of yellow gene during development. This effect may also be a form of enhancer-mediated gene expression.

References

Oct 22, 1982·Science·G M Rubin, A C Spradling
Jan 1, 1995·Trends in Genetics : TIG·M A Matzke, A J Matzke
Jun 27, 1996·Nature·A S Goldsborough, T B Kornberg
Jul 12, 1996·Cell·R Aramayo, R L Metzenberg
Sep 1, 1996·Molecular Reproduction and Development·J L Chen, F J Longo
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R MorrisC T Wu
May 14, 1999·Current Opinion in Genetics & Development·C T Wu, J R Morris

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