PMID: 7545385Sep 1, 1995Paper

Involvement of transglutaminase in the formation of covalent cross-links in the cell wall of Candida albicans

Archives of Microbiology
J Ruiz-HerreraS Mormeneo

Abstract

Activity of the enzyme glutaminyl-peptide--glutamylyl-transferase (EC 2.3.2.13; transglutaminase), which forms the interpeptidic cross-link N epsilon-(gamma-glutamic)-lysine, was demonstrated in cell-free extracts obtained from both the yeast like and mycelial forms of Candida albicans. Higher levels of enzymatic activity were observed in the cell wall fraction, whereas the cytosol contained only trace amounts of activity. Cystamine, a highly specific inhibitor of the enzyme, was used to analyze a possible role of transglutaminase in the organization of the cell wall structure of the fungus. Cystamine delayed protoplast regeneration and inhibited the yeast-to-mycelium transition and the incorporation of proteins into the cell wall. The incorporation of covalently bound high-molecular-weight proteins into the wall was sensitive to cystamine. Proteic epitopes recognized by two monoclonal antibodies, one of which is specific for the mycelial walls of the fungus, were also sensitive to cystamine. These data suggest that transglutaminase may be involved in the formation of covalent bonds between different cell wall proteins during the final assembly of the mature cell wall.

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Citations

Aug 29, 2003·Phytochemistry·Dora M RastG O Hollenstein
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Oct 26, 2013·Amino Acids·Stefano Del DucaGiampiero Cai
Jul 5, 2007·FEMS Immunology and Medical Microbiology·Alexandre Melo BailãoCélia Maria de Almeida Soares
Oct 18, 2006·FEMS Yeast Research·Salomón Bartnicki-García
May 4, 2002·Microbiology·María IranzoSalvador Mormeneo
Jan 4, 2020·World Journal of Microbiology & Biotechnology·Lovaine DuarteMarco Antônio Záchia Ayub
Jan 9, 2019·Frontiers in Pediatrics·Matthias Torsten, Lerner Aaron
Jul 18, 2020·Microorganisms·Dorota SatalaAndrzej Kozik
Feb 2, 2018·The Journal of Biological Chemistry·Elizabeth Reyna-BeltránJuan Pedro Luna-Arias
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W L ChaffinJ P Martínez

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