Involvement of Transient Receptor Potential Melastatin 7 Channels in Sophorae Radix-induced Apoptosis in Cancer Cells: Sophorae Radix and TRPM7

Journal of Pharmacopuncture
Byung Joo Kim

Abstract

Sophorae Radix (SR) plays a role in a number of physiologic and pharmacologic functions in many organs. The aim of this study was to clarify the potential role for transient receptor potential melastatin 7 (TRPM7) channels in SR-inhibited growth and survival of AGS and MCF-7 cells, the most common human gastric and breast adenocarcinoma cell lines. The AGS and the MCF-7 cells were treated with varying concentrations of SR. Analyses of the caspase-3 and - 9 activity, the mitochondrial depolarization and the poly (ADPribose) polymerase (PARP) cleavage were conducted to determine if AGS and MCF-7 cell death occured by apoptosis. TRPM7 channel blockers (Gd(3+) or 2-APB) and small interfering RNA (siRNA) were used in this study to confirm the role of TRPM7 channels. Furthermore, TRPM7 channels were overexpressed in human embryonic kidney (HEK) 293 cells to identify the role of TRPM7 channels in AGS and MCF-7 cell growth and survival. The addition of SR to a culture medium inhibited AGS and MCF-7 cell growth and survival. Experimental results showed that the caspase-3 and -9 activity, the mitochondrial depolarization, and the degree of PARP cleavage was increased. TRPM7 channel blockade, either by Gd(3+) or 2-APB or by suppressing TR...Continue Reading

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Citations

May 16, 2019·Cold Spring Harbor Perspectives in Biology·Sarah J Roberts-ThomsonGregory R Monteith
Jun 9, 2016·Journal of Clinical Nursing·Lia SousaMar Lleixà-Fortuño
Sep 1, 2013·Journal of Pharmacopuncture·Hee Jung LeeByung Joo Kim

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Methods Mentioned

BETA
electrophoresis
Assay
flow cytometry
transfection

Software Mentioned

pClamp
Microcal Origin
Origin

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