PMID: 11334620May 4, 2001Paper

Involvement of transporter recruitment as well as gene expression in the substrate-induced adaptive regulation of amino acid transport system A

Biochimica Et Biophysica Acta
R LingV Ganapathy

Abstract

We investigated the molecular mechanism involved in the adaptive regulation of the amino acid transport system A, a process in which amino acid starvation induces the transport activity. These studies were done with rat C6 glioma cells. System A activity in these cells is mediated exclusively by the system A subtype, amino acid transporter A2 (ATA2). The other two known system A subtypes, ATA1 and ATA3, are not expressed in these cells. Exposure of these cells to an amino acid-free medium induces system A activity. This process consists of an acute phase and a chronic phase. Laser-scanning confocal microscopic immunolocalization of ATA2 reveals that the acute phase is associated with recruitment of preformed ATA2 from an intracellular pool to the plasma membrane. In contrast, the chronic phase is associated with an induction of ata2 gene expression as evidenced from the increase in the steady-state levels of ATA2 mRNA, restoration of the intracellular pool of ATA2 protein, and blockade of the induction by cycloheximide and actinomycin D. The increase in system A activity induced by amino acid starvation is blocked specifically by system A substrates, including the non-metabolizable alpha-(methylamino)isobutyric acid.

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Citations

Nov 7, 2013·Pflügers Archiv : European journal of physiology·Stefan Bröer
May 14, 2003·Neurochemistry International·Tobias HeckelStefan Bröer
Dec 15, 2004·The Journal of General Physiology·Roberta R AlfieriKenneth P Wheeler
Dec 15, 2004·The Journal of General Physiology·Marçal Pastor-AngladaF Javier Casado
Oct 2, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen N JonesThomas Jansson
Oct 6, 2004·Annual Review of Nutrition·Maria HatzoglouEllen Closs
Jul 14, 2005·Annual Review of Nutrition·M S KilbergV Leung-Pineda
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Mar 23, 2010·American Journal of Physiology. Endocrinology and Metabolism·Micah J DrummondBlake B Rasmussen
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Harinder S Hundal, Peter M Taylor
Nov 15, 2012·The Journal of Clinical Endocrinology and Metabolism·Nina JanssonTheresa L Powell
May 7, 2014·PloS One·Marta TorrenteFrançois Verrey
Jan 19, 2002·Biochemical and Biophysical Research Communications·Hélène Varoqui, Jeffrey D Erickson
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·H N JonesH J McArdle
May 1, 2002·The Journal of Cell Biology·Farrukh A ChaudhryRobert H Edwards
Jun 2, 2011·Journal of Pharmaceutical Sciences·Takeo Nakanishi, Ikumi Tamai
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Dec 3, 2014·Journal of Molecular Biology·Maria G A HägglundRobert Fredriksson
Jan 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brooke D HumphreyKirk C Klasing
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Jun 1, 2006·Acta Physiologica·R Franchi-GazzolaO Bussolati

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