Involvement of TRPV4-NO-cGMP-PKG pathways in the development of thermal hyperalgesia following chronic compression of the dorsal root ganglion in rats

Behavioural Brain Research
Xin-Li DingShou-Wei Yue

Abstract

The aim of the present study was to test the hypothesis that the TRPV4-NO-cGMP-PKG cascade is involved in the maintenance of thermal hyperalgesia following chronic compression of the dorsal root ganglion (DRG) (the procedure hereafter termed CCD) in rats. CCD rats showed thermal hyperalgesia and increased nitrite production. Intrathecal administration of ruthenium red (TRPV4 antagonist, 0.1-1 nmol), TRPV4 antisense ODN (TRPV4 AS, 40 microg, daily for 7 days), N(G)-L-nitro-arginine methyl ester (l-NAME, inhibitor of NO synthase, 30-300 nmol), 1H-[1,2,4]-oxadiazolo [4,3-a] quinoxalin-1-one (ODQ, a soluble guanylate cyclase inhibitor, 50-100 nmol) or 8-(4-Chlorophenylthio) guanosine 3',5'-cyclic Monophosphothioate, Rp-Isomer sodium salt (Rp-8-pCPT-cGMPS, a PKG inhibitor, 25-50 nmol) induced a significant (P<0.001) and dose-dependent increase in the paw withdrawal latency (PWL) compared with control rats, respectively. Ruthenium red (1 nmol), TRPV4 AS (40 microg, daily for 7 days) or L-NAME (300 nmol) decreased nitrite (an index of nitric oxide formation) in the DRG of CCD rats. In addition, the phorbol ester 4alpha-phorbol 12,13-didecanoate (4alpha-PDD, TRPV4 synthetic activator, 1 nmol), co-administered with L-NAME (300 nmol), at...Continue Reading

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Citations

Oct 12, 2012·Neuroscience Bulletin·Xiao-Yu LinJun-Ling Xing
Oct 31, 2012·Journal of Cardiovascular Pharmacology·Jessica A FilosaGeraldine Rath
Dec 22, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Wei-Hsin ChenYi-Wen Lin
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Aug 10, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Juan QuShou-Wei Yue
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Apr 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Zhenyu WuChunyang Zhu
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Mar 28, 2018·Molecular Neurobiology·Hemant KumarInbo Han
Jun 25, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Jessica A Filosa, Jennifer A Iddings

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