Involvement of vacuolar proton ATPase in Junin virus multiplication

Archives of Virology
V CastillaC E Coto

Abstract

The role of vacuolar-proton ATPase (V-H+ ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A1 and concanamycin A during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in Vero and BHK-21 cells. The inhibitory action of the compounds was mainly exerted at an early stage of the JV multiplication cycle, without affecting virus attachment to the cell but preventing virus penetration. A correlation between the inhibitory action of the compounds on intracellular compartments acidification and the reduction of JV yield was observed. The addition of concanamycin A at different times after infection indicated that the compound also interferes with the release of infectious particles to the extracellular medium. Although, intracellular transport of JV glycoproteins to the cell membrane, seems not to be affected as revealed by immunofluorescence staining. The results confirm that JV enters into the cell through the endocytic pathway as previously suggested by using lysosomotr...Continue Reading

Citations

Apr 15, 2004·International Journal of Antimicrobial Agents·Vanesa C Albiol Matanic, Viviana Castilla
Sep 2, 2011·Journal of Virology·Christian D CuevasSusan R Ross
Jan 25, 2013·Viruses·Magali E Droniou-Bonzom, Paula M Cannon
Apr 18, 2014·Journal of Virology·Hideki TaniShigeru Morikawa
Feb 26, 2016·Journal of Virology·Yi-Ying ChouRaphaël Gaudin
Jun 25, 2017·Annual Review of Virology·Nicolás Sarute, Susan R Ross
Jun 20, 2019·PloS One·Maria Laura A Perez VidakovicsRicardo M Gómez

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