Involvement of ZFR1 of Fusarium verticillioides in kernel colonization and the regulation of FST1, a putative sugar transporter gene required for fumonisin biosynthesis on maize kernels.

Molecular Plant Pathology
Burton H BluhmCharles P Woloshuk

Abstract

Fumonisins comprise a class of carcinogenic mycotoxins produced by Fusarium verticillioides during colonization of maize kernels. In previous work, we identified ZFR1, which is predicted to encode a Zn(II)2Cys6 zinc finger transcription factor required for fumonisin B(1) (FB(1)) production during growth on kernels. In this study, we characterized the role of ZFR1 in colonizing maize kernels and inducing FB(1) biosynthesis. The ZFR1 deletion strain (Deltazfr1) grew approximately 2.5-fold less than the wild-type on endosperm tissue and a variety of other carbon sources, including glucose and amylopectin. However, the Deltazfr1 strain displayed higher alpha-amylase activity and expression of genes involved in starch saccharification than the wild-type, thus indicating that the reduced growth of the Deltazfr1 strain was not due to inhibition of amylolytic enzymes. In the wild-type strain, expression of six genes encoding putative sugar transporters was significantly greater on endosperm tissue than on germ tissue, and expression of at least three of the six genes was negatively affected by disruption of ZFR1. Intriguingly, disruption of FST1 had no effect on growth, kernel colonization or kernel pH but decreased FB(1) production by...Continue Reading

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Citations

Apr 9, 2013·Applied Microbiology and Biotechnology·Perng-Kuang Chang, Kenneth C Ehrlich
Dec 1, 2010·Current Genetics·Daren W Brown
Apr 23, 2009·Journal of Agricultural and Food Chemistry·Kyung MyungAna M Calvo
Feb 24, 2011·Phytopathology·Brittiney N ReeseCharles P Woloshuk
Oct 11, 2011·Applied and Environmental Microbiology·Adeline PicotFlorence Richard-Forget
Jun 21, 2012·Fungal Genetics and Biology : FG & B·Robert A E ButchkoPhilipp Wiemann
Mar 12, 2016·Applied Microbiology and Biotechnology·Sarah M RöslerBettina Tudzynski
Mar 27, 2012·Fungal Genetics and Biology : FG & B·Xianyun SunShaojie Li
Oct 20, 2012·FEMS Microbiology Reviews·Charles P Woloshuk, Won-Bo Shim
Oct 2, 2014·Molecular Plant-microbe Interactions : MPMI·Carmen Ruiz-RoldánM Isabel G Roncero
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May 20, 2016·Molecular Plant Pathology·Chenxing NiuCharles P Woloshuk
Sep 22, 2010·Molecular Plant-microbe Interactions : MPMI·Hun Kim, Charles P Woloshuk
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Feb 22, 2017·Journal of Food Protection·John B RidenourBurton H Bluhm
Apr 3, 2021·World Journal of Microbiology & Biotechnology·Fernanda AchimónJulio A Zygadlo

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