PMID: 8973378Sep 1, 1996Paper

Inward rectifier potassium channels. Cloning, expression and structure-function studies

Japanese Heart Journal
A A LagruttaJ P Adelman

Abstract

A PCR-based cloning strategy was used to identify novel subunits of the two-transmembrane domain inward rectifier potassium channel family from rat brain, heart, and skeletal muscle. When expressed in Xenopus oocytes, two of these clones (Kir4.1 and Kir2.3) gave rise to inwardly rectifying potassium currents. Two-electrode voltage clamp commands to potentials negative to EK evoked inward potassium-selective currents which rapidly reached a peak amplitude and then relaxed to a steady-state level. Differences in the extent of current relaxation, the degree of rectification, and the voltage-dependent block by external cesium were detected. Two other members of this family (Kir5.1 and Kir3.4) did not produce macroscopic currents, when expressed by themselves, yet both subunits modified the currents when coexpressed with other specific members of the Kir family. Expression of chimeric subunits between Kir4.1 and either Kir5.1 or Kir3.4 suggested that the transmembrane domains determine the specificity of subunit heteropolymerization, while the C-terminal domains contribute to alterations in activation kinetics and rectification. Expression of covalently linked subunits demonstrated that the relative subunit positions, as well as sto...Continue Reading

Citations

May 23, 1998·The Journal of Physiology·S L MironovD W Richter
May 12, 2009·Neuroscience Letters·Rikke SoeDan Arne Klaerke
Jul 21, 2009·Biochimica Et Biophysica Acta·Rikke SøeDan Arne Klaerke
Feb 9, 2008·Audiology & Neuro-otology·Jing ZouPentti Tuohimaa
Mar 28, 2017·Neurochemical Research·Alberto Pérez-SamartínRogelio O Arellano
Jul 3, 2021·International Journal of Molecular Sciences·Giulia PoliMaria Cristina D'Adamo

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