Inwardly rectifying K+ channel in retinal Müller cells: comparison with the KAB-2/Kir4.1 channel expressed in HEK293T cells

The Japanese Journal of Physiology
Y TadaY Kurachi

Abstract

Inwardly rectifying K+ (Kir) channels are considered to play the major role in the spatial buffering of glial cells. We have examined the electrophysiological properties of Kir channels in isolated rabbit Müller cells (retinal glial cells). Although a previous study reported that three kinds of Kir channels with different conductance and rectification properties were expressed in distinct regions of rabbit Müller cell membrane, we could record only a single population of Kir channels from the distal end to the endfoot in 205 successful cell-attached patches. The identified Müller cell Kir channel had a unitary conductance of 25 pS in the inward direction with symmetrical 153 mM K+ condition. The conductance and gating properties of the Müller cell Kir channels were identical to those of the KAB-2/Kir4.1 heterologously expressed in a mammalian cultured cell line, HEK293T cells. Thus KAB-2/Kir4.1 was the predominant glial Kir channel not only in the brain, but also in the retina. Because its rectification is intermediate, this Kir channel may contribute to both the intrusion and the extrusion of K+ ions across glial cell membrane and may be the major pathway for redistribution of extracellular K+ ions in the central nervous system.

References

Jan 1, 1991·Annals of the New York Academy of Sciences·A Reichenbach
Jan 1, 1990·Annual Review of Neuroscience·B A BarresD P Corey
May 1, 1987·Canadian Journal of Physiology and Pharmacology·E A Newman
Jun 1, 1988·Pflügers Archiv : European journal of physiology·B Nilius, A Reichenbach
Jan 1, 1989·Annual Review of Physiology·R LatorreO Alvarez
Jun 1, 1994·Glia·H Sontheimer
Jul 7, 1995·The Journal of Biological Chemistry·T TakumiS Nakanishi
Nov 12, 1993·Science·S R RobinsonD I Vaney
Feb 7, 1997·Science·E A Newman, K R Zahs

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Citations

Apr 21, 1999·Brain Research. Developmental Brain Research·W MaD A Stenger
Dec 15, 2000·Progress in Neurobiology·J L VeneroJ Cano
Jun 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marc PaulaisStephen J Tucker
Nov 25, 2004·Neuroscience·P Kofuji, E A Newman
Oct 26, 2001·Japanese Journal of Pharmacology·Y Horio
Nov 18, 2004·American Journal of Physiology. Renal Physiology·Daniel A GrayLawrence G Palmer
Feb 23, 2008·Glia·Yuriy V KucheryavykhSerguei N Skatchkov
Apr 11, 2003·American Journal of Physiology. Cell Physiology·Masaru IshiiYoshihisa Kurachi
Dec 30, 2014·Physiological Reviews·Francisco V SepúlvedaMaría Isabel Niemeyer
Apr 5, 2001·Journal of Neurophysiology·E SolessioE M Lasater
Jul 30, 2019·Current Eye Research·Karen EastlakeG Astrid Limb

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