Iodination of Myxococcus xanthus during development.

Journal of Bacteriology
P Maeba

Abstract

Intact cells of Myxococcus xanthus were iodinated with [125I]lactoperoxidase to permit examination of the surface components accessible to labeling during cell development. Vegetative cells, starved on a defined solid medium, aggregated, formed fruiting bodies, and produced myxospores. Cells collected at different stages were iodinated, and their proteins were analyzed by one- and two-dimensional electrophoresis and autoradiography. One-dimensional electrophoresis revealed six iodinated bands in vegetative cell extracts. During development, 10 radioactive bands were detected, 4 of which migrated to the same positions as those of vegetative cells. Only six bands were detected in purified, labeled myxospores. Of these, one band possessed mobility similar to that of labeled vegetative cell proteins, whereas the other bands possessed mobility similar to that detected in developing cells. Analysis of two-dimensional gels indicated that at least 14 proteins were iodinated in vegetative cells, one of which was intensely labeled (protein b). Another of the proteins (protein a) was labeled throughout development. During development, about 30 proteins were iodinated and the prominently labeled ones were designated c, d, e, f, and g. The ...Continue Reading

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Citations

Jan 1, 1987·Critical Reviews in Microbiology·L J Shimkets
May 1, 1992·Journal of Bacteriology·L PlamannD Kaiser
Oct 1, 1994·Journal of Bacteriology·R M Behmlander, M Dworkin
Dec 1, 1991·Journal of Bacteriology·R Glufka, P Maeba
Dec 1, 1990·Microbiological Reviews·L J Shimkets
May 1, 1986·Journal of Bacteriology·P Y Maeba

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