Iodine-124 Based Dual Positron Emission Tomography and Fluorescent Labeling Reagents for In Vivo Cell Tracking.

Bioconjugate Chemistry
Truc Thuy PhamRan Yan

Abstract

Understanding the in vivo behavior of experimental therapeutic cells is fundamental to their successful development and clinical translation. Iodine-124 has the longest half-life (4.2 days) among the clinically used positron emitters. Consequently, this isotope offers the longest possible tracking time for directly labeled cells using positron emission tomography (PET). Herein, we have radiosynthesized and evaluated two iodine-124/fluorescein-based dual PET and fluorescent labeling reagents, namely 124I-FIT-Mal and 124I-FIT-(PhS)2Mal for cell surface thiol bioconjugation. 124I-FIT-(PhS)2Mal labeled cells significantly more effectively than 124I-FIT-Mal. It conjugated to various cell lines in 22%-62% labeling efficiencies with prolonged iodine-124 retention. 124I-FIT-(PhS)2Mal mainly conjugated on the cell membrane, which was confirmed by high-resolution fluorescence imaging. The migration of 124I-FIT-(PhS)2Mal labeled Jurkat cells was visualized in NSG mice with excellent target-to-background contrast using PET/CT over 7 days. These data demonstrate that 124I-FIT-(PhS)2Mal can dynamically track cell migration in vivo using PET/CT over a clinically relevant time frame.

References

Jul 22, 2009·AJR. American Journal of Roentgenology·Jeff W M Bulte
Aug 17, 2010·Nature Medicine·Matthias T StephanDarrell J Irvine
Jan 29, 2011·Bioconjugate Chemistry·Felix F SchumacherJames R Baker
Mar 3, 2011·Molecular Pharmaceutics·Vasily V BelovMikhail I Papisov
Sep 29, 2011·Nature Reviews. Clinical Oncology·Moritz F KircherJan Grimm
Dec 1, 2012·Journal of the American Chemical Society·Ran YanErik Årstad
Sep 24, 2013·Tetrahedron Letters·Lourdes CastañedaJames R Baker
Dec 18, 2013·Immunological Reviews·Christian S Hinrichs, Steven A Rosenberg
Aug 12, 2014·Organic & Biomolecular Chemistry·Felix F SchumacherStephen Caddick
Nov 2, 2014·European Journal of Nuclear Medicine and Molecular Imaging·Putthiporn CharoenphunPhilip J Blower
Feb 24, 2015·Radiology·Noriko SatoPeter L Choyke
Apr 29, 2015·EJNMMI Research·Aditya BansalTimothy R DeGrado
Jul 4, 2015·Cell Stem Cell·Alan Trounson, Courtney McDonald
Mar 26, 2016·Frontiers in Immunology·Joseph R ScaleaWilliam Burlingham
Dec 28, 2017·Journal of the American Chemical Society·Hyunbum KimNathaniel S Hwang
May 8, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michael R WeistJohn E Shively
Nov 16, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Francis ManRafael T M de Rosales
Jan 27, 2019·American Journal of Hematology·Steven FeinsJoseph A Fraietta
Feb 9, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ramsey D BadawiSimon R Cherry
Apr 14, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Olivier MartinezSophie Papa
Feb 9, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Noriko SatoRichard W Childs

❮ Previous
Next ❯

Citations

Feb 1, 2021·Cancer Imaging : the Official Publication of the International Cancer Imaging Society·Timothy H Witney, Philip J Blower
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maxime MunchGilles Ulrich
Jul 8, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ida FribergerThuy A Tran

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging technique
fluorescence imaging
fluorescence cell imaging
ELISA
column chromatography

Software Mentioned

VivoQuant
ARRIVE

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Acta - Unio Internationalis Contra Cancrum
A F PHILLIPSG R NEWBERY
Physics in Medicine and Biology
J L HAYBITTLE
Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
Kotaro NagatsuKazutoshi Suzuki
© 2021 Meta ULC. All rights reserved