PMID: 42854Nov 1, 1979

Iodine labelling of sea anemone toxin II, and binding to normal and denervated diaphragm

Naunyn-Schmiedeberg's Archives of Pharmacology
E Habermann, L Béress

Abstract

1. Sea anemone toxin II (ATX II) which keeps the activated sodium channels open, can be labelled at its histidine residues with 125I up to a specific radioactivity of 500 Ci/mmole. Upon intraventricular injection in mice, ATX II causes acute, short-lasting hyperexcitation and convulsions. Its LD50 in mice is between 25 and 50 ng of the native peptide, and between 50 and 100 ng of the radioactive material per animal. 2. The labelled peptide is bound to mouse diaphragm from where it can be displaced by ATX II and, even better, by scorpion neurotoxin but not by other basic peptides, e.g., histone or aprotinin. Binding is not significantly influenced by 50 mM potassium, by replacing sodium with choline, by veratridine or tetrodotoxin. In contrast to binding of alpha-bungarotoxin, binding of ATX II is not changed by denervation of the diaphragm. ATX II binds not only to the muscular but also to the tendinous moiety of the mouse diaphragm. 3. ATX II lowers the surface tension of water. Further experiments are needed to establish the usefulness of 125I-ATX for labelling sodium channels in excitable membranes.

References

Nov 1, 1975·Toxicon : Official Journal of the International Society on Toxinology·L Béress, R Béress
Sep 1, 1976·European Journal of Biochemistry·G WundererW Machleidt
Mar 1, 1979·European Journal of Biochemistry·E Habermann, K Fischer
Jun 1, 1975·Toxicon : Official Journal of the International Society on Toxinology·E G LimaL Freire-Maia

Citations

May 1, 1982·Naunyn-Schmiedeberg's Archives of Pharmacology·E Habermann, G S Chhatwal
Jul 28, 1981·Biochimica Et Biophysica Acta·P AshG Wunderer
Mar 26, 1981·European Journal of Pharmacology·G Ahnert-Hilger, E Habermann
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·E Habermann, E Horváth
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·M RackH H Grünhagen
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·M Rack, G Isenberg
Jan 1, 1984·Annals of Neurology·T Narahashi
Jul 23, 1987·Biochimica Et Biophysica Acta·W SchreibmayerH A Tritthart
May 15, 1981·Analytical Biochemistry·S StengelinF Hucho

Related Concepts

Nematocyst Venoms
Denervation
Respiratory Diaphragm
Iodine Radioisotopes
Isotope Labeling, Stable
Muscle
Scorpion Venoms
Sea Anemones

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J P Vincent Lazdunski
Proceedings of the National Academy of Sciences of the United States of America
G Romey Lazdunski
Toxicon : Official Journal of the International Society on Toxinology
C Erxleben, W Rathmayer
© 2021 Meta ULC. All rights reserved