Iodine stain for detection of peptides after isoelectric focusing

Journal of Biochemical and Biophysical Methods
E GianazzaP G Righetti

Abstract

Iodine stain is used for the detection of peptides after isoelectric focusing has been developed. Ultrathin gels (240-360 micrometer) are cast and, after focusing, dried at 110 degrees C on a filter paper sheet. The paper-pasted gel is then exposed to iodine vapors for a few seconds to a few minutes, depending on the peptide load. White peptide zones are visible on a brown, uniform background. The reaction is fully reversible and can be used also for small-scale preparative purification of peptides. Better than 80% recoveries of peptide from the gel can be obtained by elution in 80% acetic acid.

References

Aug 15, 1978·Analytical Biochemistry·A GörgR Westermeier
Mar 1, 1978·Analytical Biochemistry·T Bibring, J Baxandall

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Citations

Feb 1, 1989·Journal of Biochemical and Biophysical Methods·H WajcmanP G Righetti
May 15, 1988·Analytical Biochemistry·L SeikC P Muller
Feb 1, 1987·Journal of Dental Research·W Distler, A Kröncke
Nov 7, 1980·Journal of Chromatography·P G Righetti, E Gianazza
Jun 1, 1986·Journal of Biochemical and Biophysical Methods·L SerranoJ Avila

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