PMID: 9166468Jul 1, 1995Paper

Iododeoxyuridine radiosensitization of human glioblastoma cells exposed to acute and chronic gamma irradiation: mechanistic implications and clinical relevance

The Cancer Journal From Scientific American
C J SchultzT J Kinsella

Abstract

Iododeoxyuridine (IdUrd) is a recognized sensitizer of sparsely and some densely ionizing radiations. The mechanism of sensitization remains uncertain. Sensitization is likely to involve increased production of DNA damage and/or inhibition of DNA damage repair. To test these possibilities, we have characterized acute and chronic radiosensitization, and the sublethal damage repair capacity of two human glioblastoma cell lines, with or without clinically relevant concentrations of IdUrd. Exponentially growing human glioblastoma cell lines, G18 and U251, were irradiated with acute (1.4 Gy/min) and chronic (20, 40, and 80 cGy/hr) cobalt 60 exposures in the presence and absence of 0 to 10 microM IdUrd. Clonogenic survival was determined. Sensitizer enhancement ratios and global survival curve comparisons were determined with and without IdUrd. Repair half-times for chronic exposures with and without IdUrd were calculated. Split-dose recovery following acute fractions of 5 Gy separated by 0, 0.5, 1, 2, and 5 hours was evaluated. Following acute exposure, a sensitizer enhancement ratio at 10% survival of 1.85 and 1.75 was observed at concentrations of 10 microM IdUrd for the G18 and U251 cell lines, respectively. A global comparison o...Continue Reading

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