PMID: 3754882Jun 10, 1986Paper

Iodogen-catalysed iodination of human calcitonin and Tyr(0)-katacalcin and purification of their mono- and di-iodinated derivatives by chromatofocussing

Journal of Immunological Methods
W Woloszczuk

Abstract

Synthetic calcitonin (CT) and Tyr(0)-katacalcin (tKT) were radioiodinated with Iodogen to high specific activity and with high yields. The products of the iodination procedure were chromatographed on Sephadex G-25 to remove unreacted iodide and then separated by chromatofocussing on PBE 94 (pH 9.6-6.0 for CT and pH 7.4-4.0 for tKT). Clear separation between uniodinated peptides and their mono- and di-iodinated derivatives was achieved with specific activities of 1900 and 3800 Ci/mmol for the respective mono- and di-iodinated peptides. Yields were up to 36 and 24% of mono- and di-iodinated CT and 41 and 29% for mono- and di-iodinated tKT. Our results show that Iodogen provides an effective and gentle way to iodinate peptides with high efficiency. Chromatofocussing is a simple, inexpensive and instrumentally undemanding method that can be performed without specialized chromatographic equipment and that should be applicable to a variety of different tracer preparations.

References

Mar 1, 1974·The Journal of Endocrinology·M R Redshaw, S S Lynch
Jan 1, 1970·Annual Review of Biochemistry·A N Glazer
Oct 1, 1983·International Journal of Peptide and Protein Research·E Regoeczi
Oct 29, 1982·Journal of Immunological Methods·G GhanemJ Frühling
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Oct 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C SartoriG A Tocco
Dec 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·V VergoteB De Spiegeleer

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