Ion channel expression in the developing enteric nervous system

PloS One
Caroline S HirstSonja J McKeown

Abstract

The enteric nervous system arises from neural crest-derived cells (ENCCs) that migrate caudally along the embryonic gut. The expression of ion channels by ENCCs in embryonic mice was investigated using a PCR-based array, RT-PCR and immunohistochemistry. Many ion channels, including chloride, calcium, potassium and sodium channels were already expressed by ENCCs at E11.5. There was an increase in the expression of numerous ion channel genes between E11.5 and E14.5, which coincides with ENCC migration and the first extension of neurites by enteric neurons. Previous studies have shown that a variety of ion channels regulates neurite extension and migration of many cell types. Pharmacological inhibition of a range of chloride or calcium channels had no effect on ENCC migration in cultured explants or neuritogenesis in vitro. The non-selective potassium channel inhibitors, TEA and 4-AP, retarded ENCC migration and neuritogenesis, but only at concentrations that also resulted in cell death. In summary, a large range of ion channels is expressed while ENCCs are colonizing the gut, but we found no evidence that ENCC migration or neuritogenesis requires chloride, calcium or potassium channel activity. Many of the ion channels are likely...Continue Reading

References

Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StockerO Pongs
Jan 13, 1998·Nature Genetics·E M Southard-SmithW J Pavan
Mar 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C J HearnD Newgreen
Oct 2, 2002·The Journal of Comparative Neurology·Natashka S PollockSarah McFarlane
Jan 1, 2003·The Journal of Comparative Neurology·Heather M YoungThomas Müller
Oct 1, 1954·The Journal of Comparative Neurology·C L YNTEMA, W S HAMMOND
Apr 22, 2005·The Journal of Comparative Neurology·Aaron C BartooDavid A Schneider
May 21, 2005·Mechanisms of Development·Allan M GoldsteinDrucilla J Roberts
Dec 31, 2005·American Journal of Physiology. Cell Physiology·Kyu Joo ParkKenton M Sanders
Mar 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zongming PanEdward C Cooper
Apr 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tiffany A Heanue, Vassilis Pachnis
Oct 14, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Noah R Druckenbrod, Miles L Epstein
Mar 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sharon B SannNicholas C Spitzer
Aug 14, 2008·Physiology·Albrecht SchwabChristian Stock
Sep 6, 2008·Nature Protocols·Darren J Korbie, John S Mattick
Nov 6, 2008·Developmental Neurobiology·M M HaoH M Young
Nov 28, 2008·Journal of Neurophysiology·Christa W HabelaHarald Sontheimer
Feb 28, 2009·The International Journal of Developmental Biology·Richard B Anderson
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Marlene M Hao, Heather M Young
Sep 29, 2009·Autonomic Neuroscience : Basic & Clinical·Cátia Laranjeira, Vassilis Pachnis
Oct 31, 2009·Experimental Eye Research·John L UbelsJulienne R Louters
Dec 2, 2009·Nature Reviews. Drug Discovery·Heike WulffLuis A Pardo
Jan 20, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M M HaoH M Young
Jun 17, 2010·Advances in Experimental Medicine and Biology·Andrea Becchetti, Annarosa Arcangeli
May 3, 2011·Progress in Neurobiology·Chen Gu, Joshua Barry
May 6, 2011·American Journal of Physiology. Cell Physiology·Vishnu Anand Cuddapah, Harald Sontheimer
Jul 15, 2011·Cellular and Molecular Life Sciences : CMLS·Peng-Chun Yu, Jiu-Lin Du
Oct 19, 2011·Biochemical and Biophysical Research Communications·Eva-Maria SchmidtFlorian Lang
Oct 28, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marlene M HaoPieter Vanden Berghe
Jun 8, 2012·The Journal of Physiology·Jianli Sun, Jaideep Kapur
Jul 10, 2012·Nature Neuroscience·Erik MireGuillermina López-Bendito
Aug 10, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marlene M HaoJoel C Bornstein

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
PCR
PCRs
electrophoresis

Software Mentioned

Fiji

Related Concepts

Related Feeds

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis