PMID: 7541786Mar 1, 1995Paper

Ion channels in basolateral membrane of marginal cells dissociated from gerbil stria vascularis

Hearing Research
S TakeuchiA Irimajiri

Abstract

The basolateral membrane of isolated strial marginal cells has been probed for conductive pathways by the patch-clamp technique. Two types of voltage-insensitive channels were identified in both cell-attached and excised patches. Of these, frequently (69% of excised patches) observed was a Ca(2+)-activated nonselective cation channel having a unit conductance of 24.9 +/- 0.5 pS (N = 16). Other characteristics of this type in excised patches include: 1) linear I-V relations with 150 mM K+ (pipette)/150 mM Na+ (bath), 2) a permeability sequence of NH4+ > Na+ = K+ = Rb+ > Li+, 3) a flickering block by quinine or quinidine (both 1 mM), and 4) a dose dependent block of its activity by ADP or ATP (IC50,ATP/IC50,ADP = 20-35), both from the cytosolic side. Channels with similar characteristics were found in the apical membrane of the same cell; however, the basolateral channels were 2-4 times more densely distributed than the apical counterparts. Also frequently (57%) detected was a Cl- channel of 80.0 +/- 0.5 pS (N = 6), whose activity was Ca2+ independent. Additionally, this Cl- channel had: 1) linear I-V relations with symmetric Cl-, 2) a permeability sequence of Cl- > Br- > I- > or = NO3- > or = gluconate-, and 3) a complete and re...Continue Reading

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Citations

May 30, 2001·Hearing Research·A J PaceM M Henson
May 29, 2002·Hearing Research·Philine Wangemann
Jul 12, 2003·Hearing Research·Shoichi SawadaAkinobu Kakigi
Jan 1, 1997·Hearing Research·T OshimaT Takasaka
May 1, 1997·Hearing Research·K IkedaT Takasaka
Jan 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fumiaki NinYoshihisa Kurachi
Nov 4, 2005·Cell Communication and Signaling : CCS·Daniel C MarcusPhiline Wangemann
May 16, 2009·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hiroshi Hibino
Apr 12, 2003·The Japanese Journal of Physiology·Atsuko TakamakiTakahiro Kubota
Feb 10, 2006·Hearing Research·Chunyan QuBradley A Schulte
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Toshiaki ShibataYoshihisa Kurachi
Sep 23, 2006·Physiology·Hiroshi Hibino, Yoshihisa Kurachi
Aug 3, 2007·American Journal of Physiology. Cell Physiology·Florian LangPhiline Wangemann
Feb 16, 2008·Acta Oto-laryngologica·Akinobu KakigiRie Nishioka
May 29, 2003·American Journal of Physiology. Renal Physiology·Edward C Y WangGerard Apodaca
Sep 11, 2013·Nihon Jibiinkoka Gakkai kaiho·Hiroshi HibinoYoshihisa Kurachi
Mar 15, 2019·The Journal of Physiological Sciences : JPS·Shinichi UchidaYoshihisa Kurachi
Aug 9, 1996·Neuroscience Letters·M FurukawaT Takasaka

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