PMID: 9177048Jan 1, 1997Paper

Ion channels in guard cells of Arabidopsis thaliana (L.) Heynh

Planta
M R Roelfsema, H B Prins

Abstract

Despite the availability of many mutants for signal transduction, Arabidopsis thaliana guard cells have so far not been used in electrophysiological research. Problems with the isolation of epidermal strips and the small size of A. thaliana guard cells were often prohibiting. In the present study these difficulties were overcome and guard cells were impaled with double-barreled microelectrodes. Membrane-potential recordings were often stable for over half an hour and voltage-clamp measurements could be conducted. The guard cells were found to exhibit two states. The majority of the guard cells had depolarized membrane potentials, which were largely dependent on external K+ concentrations. Other cells displayed spontaneous transitions to a more hyperpolarized state, at which the free-running membrane potential (Em) was not sensitive to the external K+ concentration. Two outward-rectifying conductances were identified in cells in the depolarized state. A slow outward-rectifying channel (s-ORC) had properties resembling the K(+)-selective ORC of Vicia faba guard cells (Blatt, 1988, J Membr Biol 102: 235-246). The activation and inactivation times and the activation potential, all depended on the reversal potential (Erev) of the s-...Continue Reading

Citations

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Feb 16, 2005·The Journal of Membrane Biology·C V MuraR Delgado
Apr 16, 2002·Trends in Plant Science·Anne Aliénor Véry, Hervé Sentenac
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Feb 1, 2001·The New Phytologist·Burkhard Sattelmacher

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