Ion fragmentation activated by matrix-assisted laser desorption/ionization in an ion-trap/reflectron time-of-flight device

Rapid Communications in Mass Spectrometry : RCM
S T FountainD M Lubman

Abstract

An ion-trap storage/reflectron time-of-flight mass spectrometer has been used to study the decay of large ions following activation by matrix-assisted laser desorption/ionization (MALDI). It is shown that large ions may undergo fragmentation over long periods of time, extending even to milliseconds in some cases. These fragments are stored in the trap and detected as stable ions in the reflectron device, rather than as metastable ions due to decay during the flight time to the detector. The ion decay is found to depend strongly on the laser intensity, while a smaller effect may be due to the matrix used in the MALDI process. The fragmentation observed was found to also depend strongly on the RF voltage applied to the ring electrode; higher RF voltage produced enhanced fragmentation. A gated RF experiment further demonstrated the importance of the level of the RF voltage, during the initial activation event, in producing fragmentation. A study of the effects of buffer gas composition and pressure showed that increased pressure may result in reduced fragmentation due to collisional cooling in the trap. However, the use of argon or nitrogen buffer gas at increased pressure in the trap may result in fragmentation upon extraction fr...Continue Reading

References

Feb 1, 1992·Rapid Communications in Mass Spectrometry : RCM·B SpenglerE Jaeger
Apr 1, 1992·Rapid Communications in Mass Spectrometry : RCM·T Cornish, R J Cotter
Dec 1, 1989·Rapid Communications in Mass Spectrometry : RCM·R C Beavis, B T Chait
Aug 1, 1987·Analytical Chemistry·P DemirevR J Cotter
Mar 1, 1993·Journal of the American Society for Mass Spectrometry·L Ngoka, C B Lebrilla

❮ Previous
Next ❯

Citations

Oct 1, 1995·Current Opinion in Structural Biology·J T Stults
May 18, 2004·Journal of the American Society for Mass Spectrometry·Yuejun ZhenJohn M Peltier
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Dec 21, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shinsaku DobashiMichikazu Hara
Sep 25, 2002·Rapid Communications in Mass Spectrometry : RCM·Wojciech Gabryelski, Liang Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.