PMID: 3768333Sep 9, 1986Paper

Ion-induced DNA structure change in nucleosomes

Biochemistry
M E HoganR Austin

Abstract

Physical methods have been used to study calcium binding to the nucleosome core particle. Equilibrium dialysis of Ca2+ and spectroscopic analysis of a Ca2+ analogue show that the ion binds tightly to the particles, resulting in a significant change of DNA circular dichroism. This suggests that base stacking may be altered as a result of Ca2+ binding. In the presence of Ca2+, the absorbance and fluorescence properties of methylene blue (MB), a DNA-specific intercalator, confirm that the dye binds tightly to nucleosomes by intercalation. However, secondary changes occur which suggest that the MB binding site is altered as a result of Ca2+ binding. Triplet state anisotropy decay and triplet lifetime quenching both show that in the Ca2+-nucleosome complex, methylene blue is capable of wobbling over a substantial angular range at its binding site. To explain these data, it is proposed that Ca2+ binding to nucleosomes causes DNA to fold by means of a series of sharp bends (kinks). The properties of bound MB are best explained if it is presumed that the intercalator binds tightly to such kinked sites in the nucleosome. On the basis of these observations, we discuss the possibility that multivalent ion concentration in the nucleus is h...Continue Reading

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Citations

Feb 20, 1990·Journal of Molecular Biology·D J Clark, T Kimura
Aug 17, 1989·Biochimica Et Biophysica Acta·J M Vanderkooi, J W Berger
Dec 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·E M Tuite, J M Kelly

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