Abstract
New methods of ion interaction reagent (IIR) RP-HPLC are presented for the determination of anti-tuberculosis drugs and their metabolites, singly or in multi-component mixtures, in biological fluids. The following analytes are considered: isoniazid, ethionamide, pyrazinamide, morphazinamide, p-aminosalicylic acid, nicotinic and isonicotinic acids. Aqueous solutions of three different ion interaction reagents are alternatively or comparatively used as the mobile phases, namely: (A) 5.00 mM octylamine at pH 3.00 for o-phosphoric acid, (B) 5.00 mM octylamine at pH 8.00 for o-phosphoric acid, and (C) 5.00 mM 1,6 diaminohexane at pH 6.00 for o-phosphoric acid. The response linearity between peak area and analyte concentration is verified for all the analytes in the concentration range within the determination limits and 2.00 mg/l. Detection limits are always lower than 82 microg/l for standard solutions; in the analysis of samples of rat serum, rat plasma and human serum, the matrix effect is negligible, the detection limits are always lower than 94 microg/l and the average recovery yield is always greater than 96%.
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Citations
Aug 31, 2013·AAPS PharmSciTech·Chintan J BhanushaliMuhammad J Habib
Dec 17, 2010·Journal of Pharmaceutical and Biomedical Analysis·Lan HongSu Zeng
Mar 6, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Priyanka A ShahPranav S Shrivastav
May 8, 2016·Talanta·Bruno Regis Lyrio FerrazAndréa Renata Malagutti
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