Ion-Ion Proton Transfer and Parallel Ion Parking for the Analysis of Mixtures of Intact Proteins on a Modified Orbitrap Mass Analyzer

Journal of the American Society for Mass Spectrometry
Scott A UgrinDonald F Hunt

Abstract

We have enabled parallel ion parking on a modified Orbitrap Elite™ as a way to control ion-ion proton transfer reactions via selective activation of a range of ions. The result is the concentration of the majority of ion current from multiple charge states of each precursor proteoform into a single charge state, maximizing signal intensity and increasing effective sensitivity compared to conventional MS1 spectra. These techniques were applied in an on-line HPLC, data-dependent MS/MS analysis of intact E. coli ribosomal proteins with HCD fragmentation. With one injection, all but two ribosomal proteins were selected for fragmentation and subsequently identified. The techniques described facilitate rapid identification of intact proteins in complex mixtures and an enhanced ability to observe proteins of low abundance.

References

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May 2, 2018·Journal of the American Society for Mass Spectrometry·David J ForemanScott A McLuckey

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Citations

Mar 17, 2021·Journal of the American Society for Mass Spectrometry·Dahang YuSi Wu
Apr 27, 2021·Journal of the American Society for Mass Spectrometry·Jake T KlineLuca Fornelli
May 19, 2020·Journal of the American Society for Mass Spectrometry·Hsi-Chun ChaoScott A McLuckey
Nov 2, 2019·Analytical Chemistry·Luis A MaciasJennifer S Brodbelt
Aug 20, 2020·Journal of the American Society for Mass Spectrometry·Kristina SrzentićMowei Zhou
Nov 7, 2019·Analytical Chemistry·David Foreman, Scott A McLuckey

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Methods Mentioned

BETA
reverse-phase chromatography

Software Mentioned

Qual Browser Xtract
XTract
IIPT
ProSight [UNK]
PIP
ProSightPC

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