Ion Mobility, Hydrogen/Deuterium Exchange, and Isotope Scrambling: Tools to Aid Compound Identification in 'Omics Mixtures

Analytical Chemistry
Hossein MalekiStephen J Valentine

Abstract

Liquid chromatography tandem mass spectrometry (LC-MS/MS), a widely used method for comparative 'omics analysis, experiences challenges with compound identification due to matrix effects, difficulty in separating isomer and isobaric ions, and long analysis times. Ion mobility spectrometry (IMS) has proven to be useful in separating isomer and isobar ions; however, IMS-MS suffers from decreased peak capacity due to the correlation in ion size and mass. In proof-of-principle experiments, the use of gas-phase hydrogen/deuterium exchange (HDX) combined with IMS-MS/MS techniques is demonstrated to offer advantages for compound identification. Measurements providing unique information for ions include m/z value, drift time in He buffer gas, drift time in He and D2O buffer gases, deuterium incorporation pattern (isotopic distribution), deuterium incorporation pattern after collisional activation, and fragment ion deuterium incorporation pattern upon collision-induced dissociation (CID). These techniques are here shown to be highly reproducible (drift time coefficients of variation < 1.0% and isotopic pattern root-mean-square deviations of 0.5-1.5%) while demonstrating an increased ability to distinguish individual molecules from diver...Continue Reading

References

Apr 1, 1991·Rapid Communications in Mass Spectrometry : RCM·V Katta, B T Chait
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T D WoodF W McLafferty
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SuckauF W McLafferty
Mar 1, 1997·Mass Spectrometry Reviews·M K Green, C B Lebrilla
Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Oct 28, 1999·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
Jul 25, 2000·Analytical Chemistry·C S Hoaglund-HyzerD E Clemmer
May 22, 2002·Journal of the American Society for Mass Spectrometry·Stephen J Valentine, David E Clemmer
Sep 13, 2002·Journal of the American Chemical Society·Jeroen A A DemmersAlbert J R Heck
Feb 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Andrew N HoofnagleNatalie G Ahn
Dec 4, 2003·Journal of the American Society for Mass Spectrometry·John C JurchenEvan R Williams
May 6, 2004·Journal of the American Chemical Society·Junmei Zhang, Jennifer S Brodbelt
Oct 7, 2005·Mass Spectrometry Reviews·Thomas E Wales, John R Engen
Jan 13, 2006·Methods in Enzymology·J Mitchell Wells, Scott A McLuckey
Sep 30, 2006·Analytical Chemistry·Amy E HilderbrandDavid E Clemmer
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Jul 8, 2008·Journal of the American Society for Mass Spectrometry·Igor A Kaltashov, Rinat R Abzalimov
May 27, 2009·Journal of the American Society for Mass Spectrometry·Rinat R AbzalimovIgor A Kaltashov
Jun 25, 2010·The Analyst·C JunotEric Ezan
Jul 19, 2008·Annual Review of Analytical Chemistry·Brian C BohrerDavid E Clemmer
Dec 22, 2010·Chemical Society Reviews·Lars KonermannYu-Hong Liu
Mar 23, 2011·Journal of Proteome Research·Stephen J ValentineDavid E Clemmer
Apr 1, 2011·The Journal of Physical Chemistry. B·Brian C BohrerDavid E Clemmer
Oct 4, 2011·International Journal of Mass Spectrometry·Thomas J KerrJohn A McLean
Nov 22, 2011·The Analyst·Aihua ZhangXijun Wang
Jul 16, 2013·Angewandte Chemie·Sebastian TittebrandtWolf D Lehmann
Jul 1, 1994·Journal of the American Society for Mass Spectrometry·E GardC B Lebrilla
May 1, 1994·Journal of the American Society for Mass Spectrometry·M E HemlingS A Carr
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates
Jul 30, 2014·Analytical Chemistry·Gregory C DonohoeStephen J Valentine
Oct 1, 2014·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Mar 24, 2015·Frontiers in Bioengineering and Biotechnology·Arnald AlonsoAntonio Julià
Apr 22, 2015·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Jul 28, 2015·Trends in Analytical Chemistry : TRAC·Arpana Vaniya, Oliver Fiehn
Dec 2, 2015·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine

❮ Previous
Next ❯

Citations

Sep 29, 2017·Journal of the American Society for Mass Spectrometry·Hossein MalekiStephen J Valentine
Sep 15, 2018·Analytical Chemistry·Abhigya MookherjeeMiklos Guttman
Sep 7, 2019·Analytical Chemistry·Yury KostyukevichEvgeny Nikolaev
Dec 4, 2021·Mass Spectrometry Reviews·Annelaure DamontJean-Claude Tabet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.