Ion mobility-mass spectrometry of charge-reduced protein complexes reveals general trends in the collisional ejection of compact subunits

The Analyst
Russell E Bornschein, Brandon T Ruotolo

Abstract

Multiprotein complexes have been shown to play critical roles across a wide range of cellular functions, but most probes of protein quaternary structure are limited in their ability to analyze complex mixtures and polydisperse structures using small amounts of total protein. Ion mobility-mass spectrometry offers a solution to many of these challenges, but relies upon gas-phase measurements of intact multiprotein complexes, subcomplexes, and subunits that correlate well with solution structures. The greatest bottleneck in such workflows is the generation of representative subcomplexes and subunits. Collisional activation of complexes can act to produce product ions reflective of protein complex composition, but such product ions are typically challenging to interpret in terms of their relationship to solution structure due to their typically string-like conformations following activation and subsequent dissociation. Here, we used ion-ion chemistry to perform a broad survey of the gas-phase dissociation of charge-reduced protein complex ions, revealing general trends associated with the collisional ejection of compact, rather than unfolded, protein subunits. Furthermore, we also discover peptide and co-factor dissociation channel...Continue Reading

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Citations

Sep 14, 2017·Analytical and Bioanalytical Chemistry·Yelena YefremovaMichael O Glocker
Mar 18, 2021·Journal of the American Society for Mass Spectrometry·Melanie Cheung See KitIan K Webb
Mar 5, 2020·Journal of the American Society for Mass Spectrometry·Micah T DonorJames S Prell

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