Ion Mobility Spectrometry and Tandem Mass Spectrometry Analysis of Estradiol Glucuronide Isomers

Journal of the American Society for Mass Spectrometry
Alana L Rister, Eric D Dodds

Abstract

Estradiol is an estrogenic steroid that can undergo glucuronidation at two different sites, which results in two estradiol glucuronide regioisomers. These isomers can be produced by different enzymes and can have different biological activities before being eliminated from the body. Although there have been previous methods that can distinguish the two isomers, these methods often have long acquisition times or high cost per analysis. In this study, traveling wave ion mobility spectrometry (TWIMS) coupled with mass spectrometry (MS) was employed to separate estradiol glucuronides using alkali metal adduction in positive ion mode, where the sodiated dimer adduct provided adequate separation both in single-component standards and in two-component mixtures. Additionally, in negative ion mode, tandem mass spectrometry (MS/MS) was used to quantitatively determine the relative composition of the two isomers. This was possible due to differences in the energetic requirements for loss of the glucuronic acid, which was characterized by energy-resolved collision-induced dissociation (CID). This work demonstrated that the intensity of the glucuronic acid neutral loss product as compared with the intensity of the intact precursor ion can b...Continue Reading

References

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Aug 1, 2012·Analytical Chemistry·Matthew F BushCarol V Robinson
Dec 4, 2016·Journal of the American Society for Mass Spectrometry·Christopher D ChouinardRichard A Yost
Nov 11, 2018·Journal of the American Society for Mass Spectrometry·Alana L RisterEric D Dodds

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Citations

Jan 14, 2020·Rapid Communications in Mass Spectrometry : RCM·Fernando VargasAlan K Jarmusch
Nov 2, 2019·Steroids·Alana L Rister, Eric D Dodds
Dec 27, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alana L Rister, Eric D Dodds
Jul 24, 2021·Journal of the American Society for Mass Spectrometry·John R F B ConnollyGordon J Dear
Sep 2, 2020·Journal of the American Society for Mass Spectrometry·Samuel W MaddoxChristopher D Chouinard

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