PMID: 7544623Aug 23, 1995Paper

Ion pair binding of Ca2+ and Cl- ions in micellar-packaged gramicidin A

Biochimica Et Biophysica Acta
N Jing, D W Urry

Abstract

The two independent NMR experiments were performed to investigate the interaction between CaCl2 and the gramicidin A (GA) ion transport channel, using 13C-enriched GA and GA molecules incorporated into dodecylphosphocholine (DPC) micelles. The chemical shifts of C-13 labeled carbonyl carbons vs. CaCl2 concentration demonstrate that Ca2+ and Cl- ions interact as an ion pair within the GA structure with the Cl- ion located near the position of the carbonyl group of the Trp11 residue some 5.5 A from the mouth of the GA helix, and the Ca2+ ion bound at the position of the carbonyl group of the Trp15 residue some 2.5 A from the entrance to the helical pore. The measurements of the 35Cl line-widths and transverse relaxation times illustrate that the interaction occurs between Cl- ions and GA in DPC when in CaCl2 solution, that no interaction is detected between Cl- ions and GA in DPC when in NaCl solution, and that the interaction between Cl- ions and GA in DPC when in MgCl2 solution is much weaker than in CaCl2 solution. In short, a Cl- ion can enter the GA when it is paired with a divalent Ca2+ ion; and Ca2+ and Cl- ions as a pair exchange rapidly with sites of the GA dimer.

References

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Citations

Nov 2, 2011·European Biophysics Journal : EBJ·Tatyana I RokitskayaYuri N Antonenko
Oct 28, 1998·Biophysical Journal·B M BurkhartW L Duax
Jul 9, 1999·Biopolymers·B M BurkhartV Pletnev
Mar 31, 2006·International Journal of Cancer. Journal International Du Cancer·Liv Tone EliassenØystein Rekdal
May 10, 2005·Rapid Communications in Mass Spectrometry : RCM·Putty-Reddy SudhirZi-Cong Zhou
Jul 10, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Eric E RossTaylor Johnston

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