Ion-transfer electrochemistry of rat amylin at the water-organogel microinterface array and its selective detection in a protein mixture

Chemistry, an Asian Journal
Eva Alvarez de EulateDamien W M Arrigan

Abstract

The behavior of proteins and polypeptides at electrified aqueous-organic interfaces is of benefit in label-free detection strategies. In this work, rat amylin (or islet amyloid polypeptide) was studied at the interface formed between aqueous liquid and gelled organic phases. Amylin is a polypeptide that is co-secreted with insulin from islet beta-cells and is implicated in fibril formation. In this study, rat amylin was used, which does not undergo aggregation. The polypeptide underwent an interfacial transfer process, from water to the gelled organic phase, under applied potential stimulation. Cyclic voltammetry revealed steady-state forward and peak-shaped reverse voltammograms, which were consistent with diffusion-controlled water-to-organic transfer and thin-film stripping or desorptive back-transfer. The diffusion-controlled forward current was greater when amylin was present in an acidic aqueous phase than when it was present in an aqueous phase at physiological pH; this reflects the greater charge on the polypeptide under acidic conditions. The amylin transfer current was concentration dependent over the range 2-10 μM, at both acidic and physiological pH. At physiological pH, amylin was selectively detected in the presen...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G J CooperK B Reid
Sep 25, 2003·Journal of the American Chemical Society·Shigeru AmemiyaTracy L Wazenegger
Sep 29, 2004·Neuron·Dominic M Walsh, Dennis J Selkoe
Sep 1, 2005·Analytical Chemistry·Jidong GuoShigeru Amemiya
Jun 27, 2007·The Analyst·Grégoire Herzog, Damien W M Arrigan
Jan 22, 2008·Analytical Chemistry·Mikhail Yu VaginMarco Mascini
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Francine KivlehanDamien W M Arrigan
Mar 24, 2009·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
May 22, 2009·Journal of the American Chemical Society·Ravi Prakash Reddy NangaAyyalusamy Ramamoorthy
May 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Toshiyuki OsakaiH Katano
Jun 11, 2010·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Aug 26, 2010·Analytical Chemistry·Rune A HartvigHenrik Jensen
Mar 11, 2011·Chemical Society Reviews·Shujuan LiuYuanhua Shao
Dec 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Rune A HartvigHenrik Jensen

Related Concepts

Electrolytes
Gel
Hydrogen-Ion Concentration
Ions
Uniport
Electrochemical Techniques
Islet Amyloid Polypeptide Precursor
Acids
Insulin
Islets of Langerhans

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