PMID: 11913460Mar 27, 2002Paper

Ion transport and ligand binding by the Na-K-Cl cotransporter, structure-function studies

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Paul Isenring, Biff Forbush

Abstract

The cation-Cl cotransporters (CCCs) mediate the coupled movement of Na and/or K to that of Cl across the plasmalemma of animal cells. Eight CCCs have been identified to date: two Na-K-Cl cotransporters (NKCC), four K-Cl cotransporters (KCCs), one Na-Cl cotransporter (NCC) and one CCC interacting protein (CIP). All of the NKCCs and KCCs are inhibited by loop diuretics; mercury and other modifying agents are also known to block NKCC-mediated transport. In this work, we have utilized a mutational approach to study the interaction between different substrates and the NKCCs. We relied on the strategy of exchanging domains between functionally distinct carriers (the shark NKCCl and the human NKCCl) to identify residues or group of residues that are involved in the interaction with ions, loop diuretics and Hg. Our results show that the N- and C-termini have no role in determining the species differences in ion transport and bumetanide binding. On the other hand, the interaction between Hg and the NKCCs is found to partially involve the C-terminus through residues that contain available sulfhydryl groups. Within the transmembrane segments, variant residues in the 2nd, 4th and 7th predicted alpha-helices are shown to encode the differen...Continue Reading

References

Nov 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·P SilvaR J Solomon
Nov 1, 1989·The Journal of Membrane Biology·J N George, R J Turner
Jan 1, 1989·Annual Review of Physiology·M Haas
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Apr 1, 1988·The Journal of Membrane Biology·R J Turner, J N George
Sep 1, 1983·The American Journal of Physiology·M Haas, T J McManus
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A Payne, B Forbush
Feb 1, 1995·The American Journal of Physiology·J Noël, J Pouysségur
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C XuB Forbush
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G GambaS C Hebert
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MatsuokaK D Philipson
Jul 5, 1996·The Journal of Biological Chemistry·J A PayneL F Donaldson
Jul 1, 1996·Journal of the American Society of Nephrology : JASN·P BlakelyD D Fanestil
Sep 26, 1997·The Journal of Biological Chemistry·P Isenring, B Forbush
Jun 6, 1998·The Journal of Biological Chemistry·P IsenringB Forbush
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·P IsenringB Forbush
Oct 12, 1999·The American Journal of Physiology·S C JacobyP Isenring
Dec 22, 1999·The American Journal of Physiology·J E RaceE J Holtzman
Jun 29, 2000·The Journal of Biological Chemistry·L CaronP Isenring

❮ Previous
Next ❯

Citations

Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masaaki AndoTomohiro Kozaka
Oct 30, 2008·The Journal of Biological Chemistry·Eva V WertheimerPablo E Visconti
Oct 24, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christopher Paul Cutler, Gordon Cramb
Oct 6, 2007·Annals of Neurology·Volodymyr I DzhalaKevin J Staley
Mar 15, 2012·Biochemical and Biophysical Research Communications·Annika DödingHans Gerd Nothwang
Nov 8, 2002·Biochimica Et Biophysica Acta·Peter W Flatman
Dec 17, 2014·CNS Neuroscience & Therapeutics·Volodymyr Dzhala, Kevin J Staley
Feb 6, 2015·Frontiers in Cellular Neuroscience·Anna-Maria Hartmann, Hans Gerd Nothwang
Dec 14, 2017·British Journal of Pharmacology·Meng-Chang KoKwok-Tung Lu
May 20, 2004·American Journal of Physiology. Renal Physiology·Claudia Tovar-PalacioGerardo Gamba
Jul 29, 2004·The Journal of Biological Chemistry·Charles F SimardPaul Isenring
Sep 7, 2004·The Journal of Biological Chemistry·Charles F SimardPaul Isenring
Jun 30, 2006·Journal of the American Society of Nephrology : JASN·Carsten A PresslerSiegfried Waldegger
Apr 21, 2006·The Journal of Biological Chemistry·Erika MorenoGerardo Gamba
Jun 6, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David H Evans
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P K LaufG A Rouleau
Aug 15, 2003·American Journal of Physiology. Cell Physiology·Tatyana A BrindikovaPaul Isenring
Jun 20, 2019·The Journal of Physiology·Andrée-Anne MarcouxPaul Isenring
Aug 18, 2017·American Journal of Physiology. Cell Physiology·A P GarneauP Isenring
Feb 26, 2014·Metabolic Brain Disease·Kakulavarapu V Rama RaoMichael D Norenberg
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Edith GagnonPaul Isenring
Feb 19, 2021·Communications Biology·Sensen ZhangMaojun Yang
Sep 25, 2003·The Journal of Experimental Biology·Ashok K PullikuthSarjeet S Gill
May 23, 2021·Journal of Molecular Biology·Thomas A ChewLiang Feng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Paul IsenringBiff Forbush
Mammalian Genome : Official Journal of the International Mammalian Genome Society
J WangT W Kurtz
© 2022 Meta ULC. All rights reserved