Ion trap mass spectrometry for kinetic studies of stable isotope labeled vitamin A at low enrichments

Analytical Chemistry
S R DuekerA J Clifford

Abstract

The role of beta-carotene in chemoprevention of cancers and other chronic diseases generated controversy when subpopulations taking beta-carotene supplements showed increased mortality in clinical trials. Determination of the dynamics of beta-carotene in individual human subjects has emerged as a high priority. Stable isotope labeled beta-carotene tracers can be employed to determine rates of conversion to retinol (vitamin A), but tracer doses must be small to minimize perturbation of endogenous retinoid and carotenoid pools. In such cases, ratios of labeled tracer/endogenous retinol are often low, and quantitative analysis at enrichments of < 1 mol% are unreliable owing to ion-molecule reactions that generate ions at the same mass as the labeled tracer even when no tracer is present. The current study demonstrates improved gas chromatography/mass spectrometry quantification of retinol-d4 and unlabeled retinol, as their tert-butyldimethylsilyl ethers, at low enrichments using an ion trap mass spectrometer operated in selected ion storage mode. Electron ionization of analyte takes place in the ion trap using conditions that eject ions outside the range m/z 390-420, and molecular ions at m/z 400 and 404 from retinol and retinol-d...Continue Reading

References

Sep 18, 1974·Journal of the American Chemical Society·V C Bulgrin, G L Lookhart
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·O B Ptitsyn

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Citations

Dec 25, 2002·The American Journal of Clinical Nutrition·Machteld van LieshoutRichard B van Breemen
Aug 25, 2004·Archives of Biochemistry and Biophysics·Betty J Burri, Andrew J Clifford

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