PMID: 7528975Dec 1, 1994Paper

Ionic currents during action potentials in mammalian skeletal muscle fibers analyzed with loose patch clamp

The American Journal of Physiology
H WoltersH B Boom

Abstract

The loose patch-clamp technique was applied to analyze transmembrane currents during propagating action potentials in superficial fibers of musculi extensor digitorum longus of the mouse in vitro. Experimentally three components were identified in the transmembrane current: 1) a capacitive, 2) an inward sodium, and 3) an outward potassium current. Other components were negligible. The capacitive current was similar in shape to the first derivative of the intracellularly measured action potential. Tetrodotoxin, tetraethylammonium, and 4-aminopyridine, applied in the pipette, were used to identify the contribution in the current by sodium and potassium ions. With extracellularly applied depolarization steps only a sodium current was observed, not a potassium current. Occasionally found outward currents were artifactual. The behaviour of delayed rectifier potassium channels in muscle fiber membranes is discussed in the light of these unexpected findings. We conclude that potassium channel activity contributing to and measured during action potential generation is in some way inaccessible to loose patch extracellular voltage-clamp stimulation and that loose patch action current recording is a useful noninvasive method to analyze me...Continue Reading

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Citations

Aug 17, 2002·British Journal of Pharmacology·Abu Zaki, R Barrett-Jolley
Nov 19, 2004·Journal of Muscle Research and Cell Motility·Jann Yee ChinChristopher L-H Huang
Aug 6, 2010·American Journal of Physiology. Cell Physiology·Benjamin L ProsserMartin F Schneider
May 9, 2013·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·I V Kubasov, R S Arutyunyan

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