Ionic currents in single smooth muscle cells of the human vas deferens

The Journal of Urology
So Young ParkSae Chul Kim

Abstract

Smooth muscle cells of the vas deferens have an important role in carrying sperms to the exterior but little is known of their electrophysiological properties. We characterized the voltage-gated ion channel currents in single smooth muscle cells of the human vas deferens (HVSMCs). We observed contractile responses of 8 circular smooth muscle strips of the human vas deferens to a high concentration (10 mM) of tetraethylammonium. HVSMCs were isolated using proteolytic enzymes (collagenase and papain), and were used for an electro-physiological study using whole cell and inside-out patch clamp configurations. The application of 10 mM tetraethylammonium induced rhythmic contractions of the strips. When HVSMCs were dialyzed with a KCl solution, step depolarizations of membrane potential evoked oscillatory outward K currents that were not inactivated. The large conductance Ca activated K (BKCa) and delayed rectifier components of the outward current were identified. The BKCa channel showed a large single channel conductance (162.7 +/- 13.2 pS with 5 mM K in the patch pipette). Two types of Ca currents were identified in the whole cell configuration. With a cell held at -50 mV an L-type Ca current was present during a depolarizing ste...Continue Reading

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Citations

Jul 18, 2006·Journal of Translational Medicine·Marco GrassoSalvatore Blanco
Jan 28, 2014·Pflügers Archiv : European journal of physiology·C H Fry, R I Jabr
Nov 3, 2009·European Journal of Pharmacology·Nnaemeka AmobiI Christopher H Smith
Jan 10, 2012·Experimental Physiology·Nnaemeka I B AmobiI Christopher H Smith
Oct 26, 2005·Drug Discovery Today·Ibrahim A Abdel-Hamid
Jul 13, 2007·Cell Calcium·Rachel Floyd, Susan Wray
Jul 26, 2011·Urologia·Angelica Grasso, Francesco Rocco
Apr 5, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyun Woo LeeYoung Chul Kim

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