Ionic influences on the phase transition of dipalmitoylphosphatidylserine

Biochemistry
R C MacDonaldE Baer

Abstract

The ionization and phase behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphoserine have been investigated under a variety of condtions by several different methods. As measured by turbidity changes, the temperature of the crystal-liquid crystal phase transition of this lipid is influenced by pH and mono- and divalent cation concentrations. The pH-transition temperature curve is congruent with the curve relating temperature to the degree of ionization of the carboxyl group of the crystalline form. The transition temperature falls from an upper plateau of 72 degrees C at low pH values, where the carboxyl group is fully protonated, to a lower plateau of 55 degrees C at high pH values, where this group is fully ionized. The apparent pK (pH at 50% ionization) of the crystalline form shifts from 6.0 to 4.6 to 3.7 with an increase of NaCl concentration from 10(-3) to 0.1 to l.0 M, respectively. These observations are in accord with a simple theoretical analysis that utilizes diffuse double layer theory and the influence of surface potential on surface concentration of protons. In qualitative terms, an increase in electrolyte concentration reduces the surface potential, the result of which is a diminution of the surface-bulk pH differenc...Continue Reading

References

Jul 3, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosG Poste
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Träuble, H Eibl
Mar 29, 1973·Biochimica Et Biophysica Acta·T Seimiya, S Ohki
Apr 16, 1973·Biochimica Et Biophysica Acta·S Batzri, E D Korn
Feb 26, 1974·Biochimica Et Biophysica Acta·D AtkinsonJ M Stubbs
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·P OverathW Stoffel
May 28, 1971·Journal of Molecular Biology·D M Engelman
Dec 1, 1971·The Journal of General Physiology·S G McLaughlinG Eisenman
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J M SteimR L Rader
Feb 2, 1971·Biochimica Et Biophysica Acta·M B Abramson
Jan 1, 1969·Biochimica Et Biophysica Acta·D Papahadjopoulos, L Weiss
Sep 6, 1958·Nature·A D BANGHAMG V SEAMAN

Citations

Dec 1, 1986·Journal of Bioenergetics and Biomembranes·C Y Kwan
Jun 29, 1979·The Journal of Membrane Biology·M A Kolber, D H Haynes
Apr 7, 1982·Biochimica Et Biophysica Acta·D BachB A Sela
Aug 26, 1991·Biochimica Et Biophysica Acta·S MassariR Colonna
Jul 20, 1977·Biochimica Et Biophysica Acta·P Comfurius, R F Zwaal
Mar 1, 1983·Chemistry and Physics of Lipids·H Vogel, M Stockburger
Jan 1, 1987·Chemistry and Physics of Lipids·J W KleinH R Petty
Mar 1, 1991·Chemistry and Physics of Lipids·H Hauser
Mar 1, 1985·Journal of Steroid Biochemistry·R GarciaA M Municio
Sep 1, 1980·Journal of Biochemical and Biophysical Methods·W S Chow, J Barber
Nov 1, 1979·Biophysical Chemistry·P Woolley, M Teubner
Jul 31, 1978·Biochimica Et Biophysica Acta·R F Zwaal
May 1, 1980·Quarterly Reviews of Biophysics·J N IsraelachviliR G Horn
Apr 1, 1981·The Journal of General Physiology·S McLaughlinA McLaughlin
Dec 30, 1987·European Journal of Biochemistry·S A Tatulian
Jan 1, 1978·Annals of the New York Academy of Sciences·D PapahadjopoulosW Pangborn
Jun 1, 1985·Cell Biophysics·B Zimmermann, D M Soumpasis
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Arun Radhakrishnan, Harden M McConnell
Nov 11, 1976·Biochimica Et Biophysica Acta·A G Lee
Jun 16, 1978·Biochimica Et Biophysica Acta·W HartmannE Sackmann
Jan 1, 1986·Membrane Biochemistry·A WalterR Blumenthal
Apr 1, 1979·European Journal of Biochemistry·G TansR F Zwaal
Jun 26, 2015·Journal of the Science of Food and Agriculture·Leqi Cui, Eric A Decker
Aug 29, 2007·Chemistry and Physics of Lipids·Andrés PuppatoM Cecilia Yappert
Mar 1, 2014·The Journal of Membrane Biology·Monika Naumowicz, Zbigniew Artur Figaszewski
Jun 16, 1978·Biochimica Et Biophysica Acta·A WattsD Marsh
Feb 12, 1980·Biochemical and Biophysical Research Communications·M J Hope, P R Cullis
Oct 10, 2006·Biophysical Journal·Marjolaine Arseneault, Michel Lafleur
Mar 18, 1991·Biochimica Et Biophysica Acta·K MatsuzakiK Miyajima
Feb 11, 2014·Biophysical Journal·Matthew J JusticeHoria I Petrache
Mar 28, 2002·Biophysical Journal·Sagar A Pandit, Max L Berkowitz
Mar 23, 1979·Biochimica Et Biophysica Acta·J S Puskin, T Martin
Mar 14, 1985·Biochimica Et Biophysica Acta·H Hauser, G G Shipley
May 23, 1976·Biochemical and Biophysical Research Communications·H HauserA Darke
Mar 3, 2004·Biophysical Journal·Horia I PetracheJohn F Nagle
Nov 5, 1999·Biophysical Journal·R C MacDonaldT J McIntosh
Sep 24, 1998·Biophysical Journal·T K RostovtsevaV A Parsegian
May 25, 2016·Scientific Reports·Bernhard FichtlMatthias F Schneider
Aug 23, 1979·Biochimica Et Biophysica Acta·K HarlosH Eibl
Nov 28, 1979·Biochemical and Biophysical Research Communications·S K Hark, J T Ho
Oct 17, 1978·Biochemistry·S MassariG Gradenigo
Feb 23, 1988·Biochemistry·L A Chung, E London
May 1, 1980·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S Suzuki, Y Akamatsu
May 15, 1978·FEBS Letters·M Egret-CharlierM Ptak
Mar 28, 2008·The Journal of Physical Chemistry. B·Andrey A Gurtovenko, Ilpo Vattulainen

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Calorimetry
Cations, Divalent
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