Ionic liquid-induced all-α to α + β conformational transition in cytochrome c with improved peroxidase activity in aqueous medium

Physical Chemistry Chemical Physics : PCCP
Pankaj BharmoriaArvind Kumar

Abstract

Choline dioctylsulfosuccinate [Cho][AOT] (a surface active ionic liquid) has been found to induce all-α to α + β conformational transition in the secondary structure of enzyme cytochrome c (Cyt c) with an enhanced peroxidase activity in its aqueous vesicular phase at pH 7.0. [Cho][AOT] interacted with Cyt c distinctly at three critical concentrations (aggregation C1, saturation C2 and vesicular C3) as detected from isothermal titration calorimetric analysis. Oxidation of heme iron was observed from the disappearance of the Q band in the UV-vis spectra of Cyt c upon [Cho][AOT] binding above C3. Circular dichroism analysis (CD) has shown the loss in both the secondary (190-240 nm) and tertiary (250-300 nm) structure of Cyt c in the monomeric regime until C1, followed by their stabilization until the pre-vesicular regime (C1 → C3). Loss in both the secondary and tertiary structure has been observed in the post-vesicular regime with the change in Cyt c conformation from all-α to α + β which is similar to the conformational changes of Cyt c upon binding with mitochondrial membrane (Biochemistry 1998, 37, 6402-6409), thus citing the potential utility of [Cho][AOT] membranes as an artificial analog for in vitro bio-mimicking. Fluoresc...Continue Reading

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Citations

Nov 4, 2015·Chemical Communications : Chem Comm·Pankaj Bharmoria, Arvind Kumar
Jan 26, 2017·MBio·David M P De OliveiraMartina L Sanderson-Smith
Oct 3, 2018·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Meena BishtJ A P Coutinho
Oct 7, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Dipak Kumar SahooHimansu S Biswal
Dec 22, 2020·Physical Chemistry Chemical Physics : PCCP·Manvir KaurTejwant Singh Kang
Jan 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Krishan KumarPannuru Venkatesu
Apr 14, 2020·The Journal of Physical Chemistry. B·Gagandeep SinghTejwant Singh Kang
May 28, 2021·The Journal of Physical Chemistry. B·Sk Saddam HossainAnunay Samanta
Feb 28, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Manvir KaurTejwant Singh Kang
Aug 25, 2020·The Journal of Physical Chemistry. B·Ashok Pabbathi, Anunay Samanta

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