Ionic liquid matrices for MALDI mass spectrometry of lignin

Analytical and Bioanalytical Chemistry
Dmitry S KosyakovNatalya S Gorbova

Abstract

The use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for the study of lignin is still extremely limited due to its low ionization efficiency. We have developed an approach for obtaining high-intensity MALDI mass spectra of lignin, based on the use of ionic liquids as matrices. Thirty-two ionic liquids consisting of large nitrogen-containing cations and anions of aromatic acids, traditionally used as crystalline matrices, were tested. It was established that ionic liquids based on N,N-diisopropyl-N-ethylammonium, N-isopropyl-N-methyl-N-tert-butylammonium, 3-aminoquinolinium, pyridinium, and 1-methylimidazolium cations and anions of ferulic, α-cyanohydroxycinnamic, and 2,5-dihydroxybenzoic acids as MALDI matrices provided high efficiency of lignin desorption/ionization with generation of singly charged protonated molecules of its oligomers. The use of such matrices in combination with the MALDI quadrupole ion trap-time-of-flight technique allows high-intensity mass spectra of lignin to be obtained without interferences from the matrix in the molecular weight range up to 3 kDa, adequately reflecting the molecular mass characteristics of lignin preparations. Using ionic liquid matrices, MS2 and MS3 MALDI...Continue Reading

References

Jan 21, 2004·Mass Spectrometry Reviews·Samantha RealeFrancesco De Angelis
Jul 11, 2006·Analytical and Bioanalytical Chemistry·Andreas Tholey, Elmar Heinzle
Aug 12, 2009·Journal of the American Society for Mass Spectrometry·Jeffrey A Crank, Daniel W Armstrong
May 15, 2010·Analytical Chemistry·Martin StrohalmVladimír Havlícek
Jun 18, 2010·Plant Physiology·Kris MorreelWout Boerjan
Aug 28, 2010·Analytical Chemistry·Kris MorreelWout Boerjan
Jul 30, 2016·Rapid Communications in Mass Spectrometry : RCM·Dmitry S KosyakovNatalia S Gorbova

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Citations

Jul 7, 2019·Analytical and Bioanalytical Chemistry·Yulin Qi, Dietrich A Volmer
Apr 15, 2020·Rapid Communications in Mass Spectrometry : RCM·Alena KubátováEvguenii I Kozliak
Jan 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruochun ZhangDietrich A Volmer
Jul 23, 2021·Mass Spectrometry Reviews·Dane R Letourneau, Dietrich A Volmer
Oct 20, 2018·Analytical Chemistry·María J Trujillo-RodríguezJared L Anderson

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