Ionic liquids as refolding additives: variation of the anion

Journal of Biotechnology
Robert BuchfinkChristian Lange

Abstract

Several ionic liquids have been shown to act as suppressors of protein aggregation and to effectively promote the in vitro refolding of denatured proteins. In this work, the influence of the anion of these organic salts on their performance as refolding enhancers was tested, using a series of N-ethyl-N'-methyl imidazolium (EMIM) salts. Variation of the anion had a profound effect on the renaturation of the recombinant plasminogen activator rPA, which served as a model protein. The effect was roughly correlated with the stability of proteins in the tested aqueous ionic liquid solutions. Strongly destabilizing anions with higher alkyl substitutions like, e.g., hexyl sulfate were unable to promote protein refolding. However, the dimethyl and diethyl phosphate salts, which are known to be quite compatible with protein stability, also effectively suppressed renaturation, while alkylated sulfate anions with the same influence on protein stability did promote in vitro refolding. Only ionic liquid co-solvent systems with an intermediate capacity for solubilizing the proteinogenic amino acid tryptophan were found to permit effective renaturation of the model protein rPA. The most effective refolding enhancer among the tested ionic liqui...Continue Reading

Citations

Feb 9, 2011·Applied Biochemistry and Biotechnology·Etsushi YamamotoTeruyuki Nagamune
Aug 27, 2013·Applied Biochemistry and Biotechnology·Sang-Woo BaeSung Ho Ha
Oct 20, 2015·International Journal of Biological Macromolecules·Maya GunchevaDenitsa Yancheva
Mar 7, 2014·Applied Biochemistry and Biotechnology·Rajan PatelAbbul Bashar Khan
Sep 12, 2012·Biotechnology Journal·Satoshi YamaguchiTeruyuki Nagamune
Aug 31, 2011·Analytical Biochemistry·Song Yi BaeT Doohun Kim
Jul 26, 2012·International Journal of Biological Macromolecules·Azadeh Fallah-BagheriAli Akbar Moosavi-Movahedi
Jun 27, 2012·International Journal of Biological Macromolecules·Mu NaushadMaroof Ali
May 1, 2012·International Journal of Biological Macromolecules·Pankaj Attri, Pannuru Venkatesu
May 10, 2016·Biomacromolecules·Stephen StrassburgDavid Hoagland
Dec 13, 2016·International Journal of Biological Macromolecules·Awanish KumarPannuru Venkatesu
Mar 10, 2017·Soft Matter·Akhil Pratap SinghSanjib Senapati
Nov 4, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Lakkoji SatishHarekrushna Sahoo
Jul 15, 2017·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Apr 7, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alesia A TietzeDiana Imhof
Nov 8, 2014·Physical Chemistry Chemical Physics : PCCP·P Madhusudhana ReddyP Venkatesu
Mar 8, 2018·Biophysics Reviews·Mouhamad Reslan, Veysel Kayser
Oct 18, 2019·Physical Chemistry Chemical Physics : PCCP·Awanish KumarPannuru Venkatesu
Nov 24, 2019·Biotechnology and Applied Biochemistry·Zeinab TakallooReza H Sajedi
Dec 15, 2010·Physical Chemistry Chemical Physics : PCCP·Pankaj AttriAnil Kumar
Jun 29, 2011·Physical Chemistry Chemical Physics : PCCP·João V RodriguesCláudio M Gomes
Nov 18, 2011·Physical Chemistry Chemical Physics : PCCP·Hermann WeingärtnerChristian Herrmann
Feb 9, 2017·Topics in Current Chemistry·Christian Schröder
Aug 2, 2017·Physical Chemistry Chemical Physics : PCCP·Pankaj AttriMasaharu Shiratani
Mar 4, 2011·Physical Chemistry Chemical Physics : PCCP·Pankaj Attri, Pannuru Venkatesu
Aug 28, 2020·Physical Chemistry Chemical Physics : PCCP·Onkar SinghHarry Bermudez
Sep 2, 2011·Physical Chemistry Chemical Physics : PCCP·Pankaj AttriNolene Byrne
Apr 30, 2013·Biotechnology and Applied Biochemistry·Azadeh Fallah-BagheriAli Akbar Saboury
Jan 1, 2014·International Scholarly Research Notices·Sophio UchaneishviliDimitri E Khoshtariya
May 5, 2018·Biophysics Reviews·Visakh V S Pillai, Antonio Benedetto
Sep 9, 2020·International Journal of Biological Macromolecules·Katarína GarajováErik Sedlák
Sep 6, 2012·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Oct 31, 2012·The Journal of Physical Chemistry. B·Marina MacchiagodenaRuggero Caminiti
Aug 10, 2013·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Oct 26, 2018·The Journal of Physical Chemistry. B·Pannuru Kiran KumarEno E Ebenso

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