Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells

The Journal of Physiology
R J EvansR A North

Abstract

1. Complementary DNAs for the ATP-gated ion channel subunits P2X1 (from human bladder) and P2X2 (from rat phaeochromocytoma (PC12) cells) were used to express the receptors in human embryonic kidney cells by stable transfection, and in Chinese hamster ovary cells by viral infection. 2. Membrane currents evoked by ATP were recorded by the whole-cell patch clamp method. The reversal potential of the current was measured with various intracellular and extracellular solutions and used to compute the relative permeability of the P2X receptor channels. 3. There was no difference between the two receptors with respect to their permeability to monovalent organic cations. The relative permeabilities (PX/PNa) were 2.3, 1.0, 1.0, 0.95, 0.72, 0.5, 0.29, 0.16, 0.04 and 0.03 for guanidinium, potassium, sodium, methylamine, caesium, dimethylamine, 2-methylethanolamine, tris(hydroxymethyl)-aminomethane, tetraethylammonium and N-methyl-D-glucamine, respectively (values for P2X2 receptor). 4. The calcium permeability of P2X1 receptors was greater than that of P2X2 receptors. Under biionic conditions (112 mM calcium outside, 154 mM sodium inside), PCa/PNa values were 3.9 and 2.2, respectively (corrected for ionic activities). 5. ATP-evoked curren...Continue Reading

Citations

Dec 3, 2013·European Journal of Pharmacology·Yuriy Pankratov, Ulyana Lalo
Jun 25, 2014·Autonomic Neuroscience : Basic & Clinical·L Camilo NavarreteJ Pablo Huidobro-Toro
Dec 26, 2001·Neuropharmacology·Miguel Díaz-HernándezM Teresa Miras-Portugal
Jul 3, 1998·European Journal of Pharmacology·K NakazawaY Ohno
Nov 19, 1997·Neuropharmacology·C VirginioA Surprenant
Feb 27, 2003·Neuropharmacology·J D ClyneR I Hume
Jun 15, 2006·The Journal of Membrane Biology·G D HousleyE N Yamoah
Jun 30, 2009·Neuromolecular Medicine·R VarmaN J Haughey
Jul 20, 2002·Biochemical and Biophysical Research Communications·Miguel Diaz-HernandezMark M Voigt
Apr 12, 2008·Purinergic Signalling·Masaru SorimachiaNorio Akaikeb
Apr 12, 2008·Purinergic Signalling·Fritz Markwardt
Apr 30, 2008·Purinergic Signalling·Manuela MoratoAntónio Albino-Teixeira
Apr 13, 2011·Purinergic Signalling·Martyn P Mahaut-SmithRichard J Evans
Sep 24, 2008·Histochemistry and Cell Biology·Christine KestlerMarion Raab
Jun 26, 1998·British Journal of Pharmacology·C Chen, R P Bobbin
Sep 2, 1998·British Journal of Pharmacology·S UenoK Inoue
Jan 30, 2002·British Journal of Pharmacology·Catherine VialRichard J Evans
Dec 16, 1998·International Urogynecology Journal and Pelvic Floor Dysfunction·C H FryG P Sui
Apr 29, 1998·Molecular Neurobiology·G D Housley
Jan 21, 2006·The Journal of Physiology·Weiyuan MaShai D Silberberg
Jun 18, 2014·Journal of Molecular Biology·Stephan Kellenberger, Thomas Grutter
Apr 27, 2004·The Journal of Physiology·Yuichiro Fujiwara, Yoshihiro Kubo
May 23, 1998·The Journal of Physiology·T Capiod
Oct 22, 2008·The Journal of Physiology·John S YoungKeith L Brain
Apr 9, 2013·Pharmacological Reports : PR·Khrystyna Yu SukhanovaDmitri V Gordienko
Jan 8, 2005·The Journal of Physiology·Thomas J HeppnerMark T Nelson
Dec 22, 2009·Trends in Neurosciences·Brian A MacVicar, Roger J Thompson
Feb 24, 2009·Trends in Neurosciences·Gary D HousleyAndreas Reichenbach
May 12, 2009·Respiratory Physiology & Neurobiology·Miyuki NagaokaToshio Hattori
Jan 19, 2000·The Journal of Physiology·S Ding, F Sachs
May 23, 2008·Neuroscience·Y PankratovA Verkhratsky
Jul 4, 2003·British Journal of Pharmacology·Vladimir S VorobjevHelmut L Haas

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